微生物共培养降解β-氯氰菊酯的适宜条件  

Appropriate conditions of β-cypermethrin degradation by a co-culture of microorganism

作　　者：苏宏南[1,2] 陈切希 赵甲元 迟原龙 贾冬英[1] 姚开[1] SU Hong-nan1,2 CHEN Qie-xi1 ZHAO Jia-yuan3 CHI Yuan-long1 JIA Dong-ying1, YAO Kai1(College of Light Industry and Food, Sichuan University, Chengdu 610065, China） 2 （Jingyang Food and Drug Administration, Deyang 618000, China） 3 （College of Life Science, Sichuan Normal University, Chengdu 610101, China)

机构地区：[1]四川大学轻纺与食品学院,四川成都610065 [2]旌阳区食品药品监督管理局,四川德阳618000 [3]四川师范大学生命科学学院,四川成都610101

出　　处：《食品与发酵工业》2018年第7期8-12,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31371775)

摘　　要：从长期喷施拟除虫菊酯类农药的菜园土壤中筛选到1株对β-氯氰菊酯具有降解能力的放线菌。生理生化及16S r DNA序列鉴定结果显示,该菌株归属于链霉菌(Streptomyces sp.K-1)。将该菌株与前期筛选的对β-氯氰菊酯具有较强降解能力的地衣芽孢杆菌B-1(Bacillus licheniformis B-1)共同培养,确定了其降解β-氯氰菊酯的适宜条件。当两种培养基的混合比例为1∶1(GSM∶LB,v/v)、菌株K-1和菌株B-1的接种比例为2∶1(v/v)、接种方式为接种菌株K-1培养24 h后再接种菌株B-1、35℃振荡(150 r/min)培养72 h时,对含量为50μg/m Lβ-氯氰菊酯的降解率可达88.3%。An actinomyce,which could degrade β-cypermethrin,was isolated from kale yard soil sprayed pyrethroid pesticides for a long time. According to physic-biochemical characteristics and 16 S r DNA sequence,the strain was classified as Streptomyces sp. K-1. Strain K-1 and Bacillus licheniformis B-1 with high degradation capacity on β-cypermethrin were co-cultured,and the appropriate degradation conditions of β-cypermethrin in the system were selected. Suitable conditions were as follows： mixed medium ratio（ GSM ∶ LB,v/v） of 1 ∶ 1,inoculation ratio（ K-1∶ B-1,v/v） of 2∶ 1,inoculating strain B-1 after inoculation of strain K-1 for 24 hours,and 35 ℃ on a rotary shaker at 150 r/min. Under these conditions,88. 3% β-Cypermethrin（ 50 μg/m L） could be removed within 72 hours.

关 键 词：β-氯氰菊酯 链霉菌K-1 地衣芽孢杆菌B-1 共培养 降解条件 

分 类 号：X172[环境科学与工程—环境科学] X592