压裂液增稠剂降解菌的化学诱变育种研究  

Study on chemical mutation breeding of fracturing fluid thickener degrading bacteria

作　　者：李慧玲[1] 程玉鹏[1] 刘祖艳[2] 赵敏[2] LI Hui-ling;CHENG Yu-peng;LIU Zu-yan;ZHAO Min(College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Life Sciences,Northeast Forestry University,Harbin 150040,Chin)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《化学工程师》2018年第7期80-82,共3页Chemical Engineer

基　　金：黑龙江省自然科学基金项目(H2015042)

摘　　要：为了获得高活性的生物酶破胶剂,降低压裂液的粘度。本研究对压裂液增稠剂降解菌枯草芽孢杆菌进行诱变。枯草芽孢杆菌分泌的β-甘露聚糖酶可以分解增稠剂,常常作为生物酶破胶剂。以Bacillus subtilis D为出发菌株,经2%浓度的甲基磺酸乙酯诱变,经过透明圈初筛和DNS复筛,获得酶活力提高的诱变菌株Bacillus subtilis D12,酶活提高了13.28%。In order to obtain high activity of biological enzyme breaker, reduce the viscosity of fracturing fluid. In this study, the fracturing fluid thickener degradation of Bacillus subtilis mutagenesis. Bacillus subtilis secreted β-mannanase can decompose thickener, often as a biological enzyme breaker. Bacillus subtilis D was used as a starting strain and mutagenized with ethyl methanesulfonate at a concentration of 2%. The mutagenized strain Bacillus subtilis D12 with enzymatic activity increased by 13.28% .

关 键 词：增稠剂 Β-甘露聚糖酶 化学诱变 

分 类 号：Q939.97[生物学—微生物学]