MG53蛋白对脂多糖诱导的小鼠海马神经元细胞氧化损伤的影响  

Effect of MG53 protein on lipopolysaccharide-induced oxidative damage in mice hippocampal neuron cells

作　　者：李鹏 马珊珊[1] 黄团结 刘雯雯 许玲 刘艳霞 刘腾飞 周建康 杨波 关方霞[1] LI Peng;MA Shanshan;HUANG Tuanjie;LIU Wenwen;XU Ling;LIU Yanxia;L;ZHOU Jiankang;YANG Bo;GUAN Fangxia(School of Life Sciences,Zhengzhou University,Zhengzhou 450001;Department of Pathology,Zheng- zhou Yihe Hospital Affiliated to Henan University,Zhengzhou 450047;Department of Neurosurgery,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学生命科学学院,郑州450001 [2]河南大学附属郑州颐和医院病理科,郑州450047 [3]郑州大学第一附属医院神经外科,郑州450052

出　　处：《郑州大学学报（医学版）》2018年第3期281-285,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目(81601078;81471306);河南省高校科技创新团队项目(15IRTSTHN022);河南省科技创新人才计划项目(154200510008);河南省国际人才合作项目(2016GH03;2016GH15);河南省重点科技攻关项目(152102310272)

摘　　要：目的:研究MG53蛋白对脂多糖(LPS)损伤的小鼠海马神经元细胞HT22的保护作用。方法:用不同质量浓度(25、50、100、250、500、1 000、1 500 mg/L)LPS分别处理HT22细胞12、24、36、48、60 h后,CCK-8法检测细胞增殖,筛选LPS的作用时间及浓度。将HT22细胞分为正常对照组、MG53(30 mg/L)组、LPS组和MG53+LPS组,分别采用CCK-8法、Annexin V-FITC/PI双染法、流式细胞术和划痕实验检测各组细胞存活率、凋亡、细胞周期和迁移距离,ELISA法检测细胞内SOD活性和MDA含量,采用Western blot法检测Bax、Bcl-2和Cyclin D1蛋白的表达。结果:经筛选,选定以500 mg/L的LPS作用48 h为细胞模型的实验条件。MG53可降低HT22细胞的凋亡率,促进G0/G1期细胞向S期转运,增加其迁移距离;升高细胞内SOD活性,降低MDA含量,增加细胞内Bcl-2和Cyclin D1蛋白表达,降低Bax蛋白表达(P<0.05)。结论:MG53蛋白对LPS诱导的HT22细胞氧化损伤具有保护作用。Aim： To investigate the improved effect of MG53 protein on lipopolysaccharide（ LPS）-induced damage in mice hippocampal neuron HT22 cells. Methods： HT22 cells were treated with different concentrations（ 25,50,100,250,500,1 000,1 500 mg/L） of LPS for 12,24,36,48 and 60 h,and CCK-8 assay was used to detect proliferation. HT22 cells were allocated into four groups： normal control group,MG53（ 30 mg/L） group,LPS group and MG53 ＋ LPS group.The proliferation,apoptosis,cell cycle and migration distance were detected by CCK-8 assay,Annexin V-FITC/PI,flow cytometry and Transwell assay,respectively,SOD and MDA were measured by ELISA kit,and the expressions of Bax,Bcl-2 and Cyclin D1 protein were detected by Western blot. Results： The cell proliferation inhibition rate of HT22 cells treated by 500 mg/L LPS for 48 h was close to 50%,so the experimental condition was determined. migration distance,cells in S phase of the HT22 cells treated by MG53 increased significantly,while apoptosis rate and cells in G0/G1 phase decreased significantly（ P〈0. 05）; SOD activity increased but MDA content decreased; meanwhile,the expression of Bcl-2 and Cyclin D1 protein increased,and Bax expression decreased significantly（ P〈0. 05）. Conclusion： MG53 protein could improve the LPS-induced oxidative damage in HT22 cells.

关 键 词：MG53蛋白 海马神经元 氧化损伤 小鼠 

分 类 号：R741.05[医药卫生—神经病学与精神病学]