四种人子宫内膜癌细胞系中IGFBP-rP1的定位及表达  

Localization and expression of IGFBP-rP1 in four human endometrial carcinoma cell lines

作　　者：雷冬梅[1] 赵晨阳 郭瑞霞[2] 雷佳[2] 楚天骄[1] 林艳丽 LEI Dongmei;ZHAO Chenyang;GUO Ruixia;LEI Jia;CHU Tianjiao;LIN Yanli(Department of Pathology,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Department of Gynecology and Obstetrics,the First Affiliated Hospital,Zhengzhou University,Zheng- zhou 450052)

机构地区：[1]郑州大学第三附属医院病理科,郑州450052 [2]郑州大学第一附属医院妇产科,郑州450052

出　　处：《郑州大学学报（医学版）》2018年第3期309-312,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目(31670844);河南省高校科技创新团队支持计划项目(17IRTSTHN021)

摘　　要：目的:检测不同子宫内膜癌细胞系中胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-r P1)的定位及表达情况。方法:体外培养子宫内膜癌细胞Ishikawa、RL95-2、HEC-1B和HEC-1A,细胞免疫荧光法检测IGFBP-r P1在4种子宫内膜癌细胞中的定位;实时荧光定量PCR法与Western blot法检测4种子宫内膜癌细胞中IGFBP-r P1 mRNA及蛋白的表达水平。结果:细胞免疫荧光染色示IGFBP-r P1主要定位于4种子宫内膜癌细胞的胞浆。子宫内膜癌Ishikawa、RL95-2细胞中IGFBP-r P1 mRNA及蛋白表达水平明显高于HEC-1A、HEC-1B细胞(P<0.001)。HEC-1A细胞中IGFBP-r P1 mRNA及蛋白表达量最低,Ishikawa细胞中表达量最高(P均<0.05)。结论:4种子宫内膜癌细胞系中均表达IGFBP-r P1。Aim： To detect the expression of insulin-like growth factor binding protein-related protein 1（ IGFBP-rP1）in different human endometrial carcinoma cell lines. Methods： The human endometrial carcinoma cell lines Ishikawa,RL95-2,HEC-1 B and HEC-1 A were cultured in vitro. The localization of IGFBP-rP1 in 4 different endometrial carcinoma cell lines was detected by cell immunofluorescence. Using fluorescence quantitative PCR and Western blot,we examined the mRNA and protein expression of IGFBP-rP1 in 4 endometrial carcinoma cell lines. Results： Immunofluorescence staining showed that IGFBP-rP1 was localized in the cytoplasm of 4 endometrial cancer cell lines. The expression of IGFBP-rP1 mRNA and protein in Ishikawa and RL95-2 cells were significantly higher than that in HEC-1 A and HEC-1 B cells（ P〈0. 001）. IGFBP-rP1 mRNA and protein had the lowest expression in HEC-1 A cells and the highest expression in Ishikawa cells（ P〈0. 05）. Conclusion： IGFBP-rP1 is expressed in 4 human endometrial carcinoma cell lines.

关 键 词：子宫内膜肿瘤 胰岛素样生长因子结合蛋白相关蛋白1 胰岛素样生长因子 子宫内膜癌细胞系(Ish-ikawa RL95-2 HEC-1B和HEC-1A) 

分 类 号：R737.33[医药卫生—肿瘤]