血管紧张素Ⅱ对肝星状细胞蛋白激酶Cε、Cα表达的影响  

Effects of angiotensin Ⅱ on protein kinase Cε and Cα expression in hepatic stellate cells

作　　者：秦文燕 许春芳[2] 林琪 宋章章 宋凌云 李国军 Qin Wen-yah1,2; Xu Chunfang2; Lin Qi1; Song Zhangzhang3;Song Lingyun1; Li Guojun1(1. Department of Gastroenterology, the Second Hospital of Yinzhou, Ningbo 315040, China ; 2.The First affiliated Hospital of Suzhou University, Suzhou 215006, China ; 3.Medicine School of Ningbo University, Ningbo 315200, Chin)

机构地区：[1]宁波市鄞州区第二医院消化科,315040 [2]苏州大学附属第一医院消化科,215006 [3]宁波大学医学院,315200

出　　处：《中国医师杂志》2018年第7期995-998,共4页Journal of Chinese Physician

基　　金：浙江省医药卫生一般研究项目(2016KYA162);宁波市科技重大专项肝病基金(2016C51008)~~

摘　　要：目的探讨血管紧张素Ⅱ对肝星状细胞蛋白激酶Cε(PKCε)、Cα(PKCα)表达的影响。方法采用不同浓度的血管紧张素Ⅱ处理HSC-T6细胞株,四唑盐(MTT)法检测HSC-T6细胞增殖,分析羟脯氨酸含量变化,免疫荧光染色检测PKCe和PKCα的表达,RT-PCR分析PKCε和PKCαmRNA的水平。结果不同浓度血管紧张素Ⅱ促进HSC-T6细胞增殖(F=25.321,P<0.001),上调羟脯氨酸水平(F=13. 283,P <0. 001),并呈现明显的剂量效应;伴随血管紧张素Ⅱ浓度的增加,PKCε表达明显增加(F=21.387,P<0.01),并发生转位于细胞膜;PKCα表达明显增加,尤以转位膜、胞浆比较明显(F=19.431,P <0. 01),并呈现明显的剂量效应;同时PKCε和PKCαmRNA水平增加(F=13. 279、15. 174,P<0.05)。结论血管紧张素Ⅱ能够剂量依赖性地上调肝星状细胞胶原表达,提高蛋白激酶Cε、Cα表达水平,促进肝星状细胞的增殖。Objective To investigate the effect of angiotensin Ⅱ on protein kinase Cε （PKCε） and protein kinase Cα （PKCα） expression in hepatic stellate cells. Methods Hepatic stellate cell （HSC）-T6 cells were treated with different concentrations of angiotensin Ⅱ and the proliferation of HSC-T6 cells was de- tected by methyl thiazolyl tetrazolium （MTT） assay. The expression of PKCε and PKCoL was detected by immunofluorescence staining. PKCε and PKCα mRNA levels was detected by real time polymerase chain re- action （PCR）. Results Angiotensin Ⅱ concentrated the proliferation of HSC-T6 cells and the level of hydroxyproline （F =25. 321, 13. 283, P 〈0. 001 ） and showed a dose-dependent effect. With the increase of angiotensin Ⅱ concentration, PKCε significantly increased and translocated in the cell membrane; PKCα increased significantly, especially in transplanted membrane and cytoplasm （F = 21. 387, 19. 431, P 〈 0. 01 ） , and showed obvious dose effect. Meanwhile, Angiotensin Ⅱ increased the expression of PKCε and PKCα, and induced cell proliferation by up-regulating PKCε and PKCα mRNA levels （F = 13. 279, 15. 174, P 〈 0. 05 ）. Conclusions Angiotensin Ⅱ can up-regulate the expression of collagen in hepatic stel- late cells in a dose-dependent manner, increase the expression of protein kinase Cε and Cα, and promote the proliferation of hepatic stellate cells.

关 键 词：血管紧张素Ⅱ/药理学 肝星形细胞/药物作用/酶学 蛋白激酶Cα/代谢 蛋白激 酶Cε/代谢 细胞增殖 

分 类 号：R575[医药卫生—消化系统]