Therapeutic Role of Tangshenkang Granule(糖肾康颗粒) in Rat Model with Diabetic Nephropathy  

作　　者：HU Shun-jin SHU Bing JIN Hua LI Xiao-feng MAO Jia-rong REN Ke-jun GAO Lei YANG Li WU You-wan WANG Yong-jun 

机构地区：[1]Department of Internal Medicine,the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine [2]Department of Orthopaedics,Longhua Hospital,Shanghai University of Traditional Chinese Medicine [3]Spine Research Institute,Shanghai University of Traditional Chinese Medicine [4]Department of Pathology,Shanxi Hospital of Traditional Chinese Medicine [5]Graduate School,Anhui University of Traditional Chinese Medicine

出　　处：《Chinese Journal of Integrative Medicine》2018年第8期600-605,共6页中国结合医学杂志（英文版）

基　　金：Supported by the Scientific Research Project of Colleges and Universities in Anhui Province,China(No.2013z172);the National Natural Science Foundation of China(No.81503590)

摘　　要：Objective： To evaluate the renal protective effect of Tangshenkang Granule（糖肾康颗粒） in a rat model of diabetic nephropathy（DN）.Methods： Forty male Sprague-Dawley rats were randomly divided into control,DN,Tangshenkang and benazepril groups.DN model was established in the rats of DN,Tangshenkang and benazepril groups.Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks,respectively.Urinary albumin and creatinine were detected.The albumin/creatinine（ACR） was calculated in addition to 24 h urinary protein（24-h UPr）,serum creatinine（Scr）,blood urea nitrogen（BUN）,total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL）,and creatinine clearance rate（Ccr）.Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining.The average glomerular diameter（DG）,average glomerular（AG） and mesangial areas（AM） were measured.The thickness of glomerular basement membrane（TGBM） was detected using transmission electron microscope.Results： Compared with rats in the control group,rats in the DN group showed significantly decreased body weight,increased hypertrophy index,24-h urinary volume,24-h UPr,ACR,Scr,BUN,Ccr,blood lipids as well as renal pathological indices including DG,AG,AM,AM/AG and TGBM（P〈0.05）.Compared with the DN group,the weights of rats in the Tangshenkang and benazepril groups were significantly increased,and the renal hypertrophy indices were significantly decreased（P 〈0.05）.The 24-h urinary volumes,ACR,24-h UPr,Scr,BUN,Ccr,LDL,DG,AG,AM and TGBM were obviously decreased（P 〈0.05）.Compared with the benazepril group,the Tangshenkang group showed significantly decreased levels of ACR,24-h UPr,AG and AM（P 〈0.05）.Conclusion： Tangshenkang Granule decreased the urinary protein,attenuated the high glomerular filtration ratObjective： To evaluate the renal protective effect of Tangshenkang Granule（糖肾康颗粒） in a rat model of diabetic nephropathy（DN）.Methods： Forty male Sprague-Dawley rats were randomly divided into control,DN,Tangshenkang and benazepril groups.DN model was established in the rats of DN,Tangshenkang and benazepril groups.Tangshenkang Granule solution and benazepril hydrochloride solution were intragastrically administered daily to the rats in the Tangshenkang and benazepril groups for 8 weeks,respectively.Urinary albumin and creatinine were detected.The albumin/creatinine（ACR） was calculated in addition to 24 h urinary protein（24-h UPr）,serum creatinine（Scr）,blood urea nitrogen（BUN）,total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein（LDL）,high-density lipoprotein（HDL）,and creatinine clearance rate（Ccr）.Right kidneys were harvested for pathological observation using periodic acid-silver methenamine-Masson staining.The average glomerular diameter（DG）,average glomerular（AG） and mesangial areas（AM） were measured.The thickness of glomerular basement membrane（TGBM） was detected using transmission electron microscope.Results： Compared with rats in the control group,rats in the DN group showed significantly decreased body weight,increased hypertrophy index,24-h urinary volume,24-h UPr,ACR,Scr,BUN,Ccr,blood lipids as well as renal pathological indices including DG,AG,AM,AM/AG and TGBM（P〈0.05）.Compared with the DN group,the weights of rats in the Tangshenkang and benazepril groups were significantly increased,and the renal hypertrophy indices were significantly decreased（P 〈0.05）.The 24-h urinary volumes,ACR,24-h UPr,Scr,BUN,Ccr,LDL,DG,AG,AM and TGBM were obviously decreased（P 〈0.05）.Compared with the benazepril group,the Tangshenkang group showed significantly decreased levels of ACR,24-h UPr,AG and AM（P 〈0.05）.Conclusion： Tangshenkang Granule decreased the urinary protein,attenuated the high glomerular filtration rat

关 键 词：diabetic nephropathy Tangshenkang Granule kidney function lipid metabolism kidneypathology Chinese medicine 

分 类 号：R-332[医药卫生] R587.2R692.9