endocan在脓毒症致早期急性呼吸窘迫综合征小鼠肺泡灌洗液中的表达  

Expression of endocan in bronchoalveolar lavage fluid in sepsis induced early acute respiratory distress syndrome mouse model

作　　者：吴烨明 顾洁 袁彩云 李军[1] 缪红军[1] WU Yeming, GU Jie, YUAN Caiyun, LI Jun, MIAO Hongjun(The Affiliated Children＇s Hospital of Nanjing Medical University, Nanfing 210008, Chin)

机构地区：[1]南京医科大学附属儿童医院,江苏南京210008

出　　处：《现代医学》2018年第5期510-513,共4页Modern Medical Journal

基　　金：江苏省科技厅临床医学科技专项(BL2014007)

摘　　要：目的:检测脓毒症致早期急性呼吸窘迫综合征(ARDS)小鼠肺泡灌洗液中endocan的表达。方法:将40只SPF级6~8周的C57BL/6小鼠随机分为实验组(CLP组)和对照组(Sham组),分别进行盲肠结扎穿孔术(CLP)和假手术,术后24 h收取小鼠肺泡灌洗液,采用Elisa法检测endocan、肿瘤坏死因子(TNF-α)、白介素-6(IL-6)、白介素-18(IL-18)、干扰素-γ(IFN-γ)的含量,并分析endocan与IFN-γ、IL-6、IL-18、TNF-α之间的相关性。结果:CLP组小鼠肺泡灌洗液中endocan、IL-6、IL-18、TNF-α、IFN-γ的含量明显高于Sham组,差异均有统计学意义(P<0.05)。endocan与IFN-γ、IL-6、IL-18、TNF-α水平呈正相关(均P<0.05)。结论:脓毒症致早期ARDS小鼠肺泡灌洗液中endocan表达显著升高,提示endocan可作为脓毒症致早期ARDS潜在的生物标志物。Objective： To establish sepsis induced early acute respiratory distress syndrome（ ARDS） mouse model by cecal ligation and puncture（ CLP） and to observe the expression of endocan in the bronchoalveolar lavage fluid（ BALF）. Methods： Forty SPF C57 BL/6 mouse aged 6-8 weeks were randomly divided into two groups,experimental group（ CLP group） and control group（ Sham group） for CLP and sham operation respectively. After24 h,the levels of endocan,IL-6,IL-18,TNF-α and IFN-γ in BALF were measured by Elisa kit. The correlation between endocan and IL-6,IL-18,TNF-α,IFN-γ were analyzed. Results： Compared with Sham group,the levels of endocan,IL-6,IL-18,TNF-α and IFN-γ in BALF in the CLP group were significantly higher（ P〈0. 05）. All the difference were statistically significant on the expression. The levels of endocan had significant positive correlation with IL-6,IL-18,TNF-α,IFN-γ respectively. Conclusion： The endocan expression in BALF is increased in mouse with early ARDS induced by sepsis,suggesting that endocan may be a potential biomarker for early ARDS caused by sepsis.

关 键 词：脓毒症 早期ARDS endocan 小鼠 

分 类 号：R-33[医药卫生] R563.8