ciRS-7在食管癌组织中的表达及其对食管癌细胞TE1生物学特性的影响  

Expression of circular RNA ciRS-7 in tissues of sophageal squamous cell carcinoma and its effect on cell biological characteristics of TE1 cells

作　　者：孟令娇 丁平安 巨英超[3] 刘飞[1] 刘世娜 刘思桦 常胜 谷丽娜[1] 桑梅香[1] MENG Lingjiao;DING Pingan;JU Yingchao;LIU Fei;LIU Shina;LIU Sihua;CHANG Sheng;GU Lina;SANG Meixiang(a.Research Cente;b.The Third Department of Surger;c.Laboratory Animal Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,Chin)

机构地区：[1]河北医科大学第四医院科研中心,河北石家庄050011 [2]河北医科大学第四医院普通外科三科,河北石家庄050011 [3]河北医科大学第四医院实验动物中心,河北石家庄050011

出　　处：《中国肿瘤生物治疗杂志》2018年第7期726-732,共7页Chinese Journal of Cancer Biotherapy

基　　金：河北省自然科学基金资助项目(No.2016206410)~~

摘　　要：目的:探讨环状RNA(circ RNA)ciRS-7在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中的表达及对ESCC细胞株增殖、迁移和侵袭的影响。方法:选取河北医科大学第四医院2016年5月至2017年4月收治的60例食管癌患者病理组织标本及配对癌旁组织,通过q RT-PCR检测ciRS-7的表达水平。过表达或者敲低ciRS-7后,采用CCK-8法检测ESCC细胞株TE1的增殖情况,同时采用划痕实验和Transwell实验分别检测细胞迁移和侵袭能力的变化。最后通过动物实验在体内进行验证。结果:ciRS-7在ESCC组织中高表达,且表达水平与病理分级和淋巴结转移有关(均P<0.05)。过表达ciRS-7后,ESCC细胞株TE1的增殖、迁移和侵袭能力显著升高(均P<0.05);沉默ciRS-7后,TE1细胞的增殖、迁移和侵袭能力均显著降低(均P<0.05)。动物实验结果显示,转染ciRS-7表达质粒组裸鼠的肿瘤体积和质量均明显高于空载体组(均P<0.05)。免疫组化检测结果表明,转染ciRS-7表达质粒组裸鼠的肿瘤组织中增殖相关抗原(ki67、PCNA)、转移相关抗原(MMP2、MMP9)表达明显高于空载体组(均P<0.05)。结论:ciRS-7在食管癌组织中表达上调,并且会增强食管癌细胞的增殖、迁移和侵袭能力,提示ciRS-7可以作为诊断和治疗食管鳞状细胞癌的潜在作用靶点。Objective： To investigate the expression of ciRS-7 in esophageal squamous cell carcinoma （ESCC） and its effect on the cellular proliferation, migration and invasion. Methods： The cancer tissues and paired adjacent normal tissues from 60 ESCC patients treated in the Fourth Hospital of Hebei Medical University between May, 2016 and April, 2017 were selected for this study. The expressions of ciRS-7 were detected by qRT-PCR. After over-expressing or silencing of ciRS-7, the proliferation of ESCC cell line TE1 was measured by CCK-8 assay; and the migration and invasion were tested by wound healing assay and Transwell invasion assay,respectively.Finally, the effect was validated via animal experiment. Results： CiRS-7 was highly expressed in ESCC tissues （P〈0.05）, and its expression level was closely related to pathological grade and lymph node metastasis （P〈0.05）. Over-expression of ciRS-7 significantly increased the proliferation, migration and invasion （all P〈0.05） of TE1 cells; while silencing of ciRS-7 remarkably suppressed the proliferation,migration and invasion （all P〈0.05）. Conclusion： CiRS-7 was up-regulated in ESCC and could enhance ESCC cell proliferation,migration and invasion, suggesting that ciRS-7 could be used as a potential target for the diagnosis and treatment of ESCC.

关 键 词：ciRS-7 食管鳞状细胞癌(ESCC) 增殖 TE1 细胞 迁移 侵袭 

分 类 号：R735.1[医药卫生—肿瘤]