极北鲵消化道6种内分泌细胞的免疫组织化学研究  

Immunohistochemical Study on Six Kinds of Endocrine Cells in the Digestive Tract of Salamandrella keyserlingii

作　　者：赵丽丽 任春宇[1] 李淑兰[1] ZHAO Li-Li;REN Chun-Yu;Ll Shu-Lan(School of Life Sciences and Technology,Harbin Normal University,Harbin 150025,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《动物学杂志》2018年第4期589-596,共8页Chinese Journal of Zoology

基　　金：黑龙江省自然科学基金项目(No.C201219)

摘　　要：应用卵白素-生物素-过氧化物酶复合物(ABC)免疫组织化学法,对极北鲵(Salamandrella keyserlingii)消化道5-羟色胺(5-HT)、胃泌素(Gas)、生长抑素(SS)、胰高血糖素(Glu)、人胰多肽(PP)和P-物质(SP)6种内分泌细胞的分布进行了研究。文中采用Duncan多重比较的方法,对消化道内分泌细胞的分布密度进行统计学分析。结果显示,5-羟色胺细胞在消化道各部位均有分布,胃体(2.80±0.70)和十二指肠(2.60±0.75)分布密度最高,幽门最低(0.85±0.67);胃泌素细胞仅分布于小肠,十二指肠(1.85±0.75)密度最高;生长抑素细胞分布于食管、胃体、幽门、十二指肠和回肠各部位,且幽门(2.25±0.64)分布密度最高;胰高血糖素细胞只见于胃贲门部和胃体,人胰多肽细胞和P-物质细胞在整个消化道均未检测到。各种内分泌细胞的形态多样,呈圆形、椭圆形、锥体形和梭形等。极北鲵消化道内分泌细胞的这种密度分布和形态特点与其他两栖类动物及黑龙江产地的极北鲵相比,既有共性又有差异,其原因可能与物种、食性和栖息地环境等因素有关。Distribution patterns of six kinds of endocrine cells,i.e.cells secreting 5-hydroxtryptamine（5-HT）,gastrin（Gas）,somatostatin（SS）,slucagon（Glu）,pancreatic polypeptide（PP） and substance P（SP）,in the digestive tract of Salamandrella keyserlingii were investigated using the avidin-biotin-peroxidase complex（ABC） immunohistochemical method.Statistical analysis of distribution density of endocrine cells in the digestive tract was conducted following Duncan methods.The results showed that 5-HT cells were distributed in all parts of the digestive tract,with the highest density in gastric body（2.80±0.70） and duodenum（2.60±0.75）,but the lowest in the pylorus（0.85±0.67）;Gas cells were merely distributed in small intestine with their highest density distributed in duodenum（1.85±0.75）;SS cells were distributed in the esophagus,stomach body,pylorus,duodenum and ileum,with their highest density distributed in pylorus（2.25±0.64）;Glu cells were merely found in gastric cardia and gastric body;PP and SP cells were not detected in the whole digestive tract（Table 2）.The shapes of various endocrine cells were round,oval,cone-shaped and fusiform（PlateⅠ）.The density distribution and morphology of endocrine cells in the digestive tract were similar to those of Salamandrella keyserlingii and other amphibians inhabited in Heilongjiang province,yet unique characteristics of S.keyserlingii existed,and the reason of which may be due to factors such as food sources and habitats.

关 键 词：极北鲵 消化道 内分泌细胞 免疫组织化学 

分 类 号：Q955[生物学—动物学]