血管内皮生长因子基因-460T〉C位点多态性与支气管肺发育不良的相关性分析  

The correlation analysis between vascular endothelial growth factor gene polymorphism （-460T〉C）and bronchopulmonary dysplasia

作　　者：杜巧燕[1] 梅花[1] 张钰恒 新春 贾昕[1] Du Qiaoyan;Mei Hua;Zhang Yuheng;Xin Chun;Jia Xin(Department of Neonatology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院新生儿科,呼和浩特010050

出　　处：《中华新生儿科杂志（中英文）》2018年第4期241-245,共5页Chinese Journal of Neonatology

基　　金：内蒙古自然科学基金项目[2015MS（LH）0810];内蒙古医科大学附属医院重大科技项目（NYFYZD008）;内蒙古医科大学青年创新基金（YKD2015QNCX040）

摘　　要：目的探讨血管内皮生长因子（vascular endothelial growth factor，VEGF）基因－460T〉C位点多态性及其与早产儿支气管肺发育不良（bronchopulmonary dysplasia, BPD）的相关性。方法前瞻性选择2014年10月至2017年10月内蒙古医科大学附属医院新生儿科收治的支气管肺发育不良早产儿为BPD组；同期选择同民族非BPD早产儿为对照组，BPD组与对照组纳入比例为1∶1。采用聚合酶链反应扩增基因分析技术检测VEGF基因－460T〉C位点的基因型和等位基因分布。结果共纳入BPD组早产儿100例，其中蒙古族和汉族各50例；对照组100例。两组早产儿VEGF基因－460T〉C位点上均可检测出TT和CT 2种基因型。BPD组2种基因型频率分别为38.0%和62.0%，C等位基因频率为31.0%，T等位基因频率为69.0%。对照组2种基因型频率分别为29.0%和71.0%，C等位基因频率为35.5%，T等位基因频率为64.5%。两组等位基因和基因型频率差异均无统计学意义（P＝0.178、0.338）。BPD组蒙古族2种基因型频率分别为40.0%和60.0%，C等位基因频率为30.0%，T等位基因频率为70.0%；汉族2种基因型频率分别为36.0%和64.0%，C等位基因频率为32.0%，T等位基因频率为68.0%。BPD组蒙古族与汉族等位基因和基因型频率差异亦无统计学意义（P＝0.680、0.648）。结论VEGF基因－460T〉C位点多态性与内蒙古地区早产儿BPD的发生及发展无明显相关性。ObjectiveTo study the polymorphism of vascular endothelial growth factor （VEGF） gene-460T〉C locus and its correlation with bronchopulmonary dysplasia （BPD） in preterm infants.MethodFrom October 2014 to October 2017, a prospective study was conducted on BPD preterm infants admitted to the neonatal department of the Affiliated Hospital of Inner Mongolia Medical University. During the same time, non-BPD preterm infants of the same nation and same ethnic group were selected as the control group. The ratio between the BPD group and the control group was 1∶1. Genotype and allelic distribution of VEGF-460T〉C loci in both groups were detected using polymerase chain reaction method.ResultA total of 100 BPD infants were enrolled in the BPD group, including 50 cases of Mongolian nationality and 50 cases of Han nationality. Both TT and CT genotypes were detected in the VEGF-460T〉C locus in the BPD and control groups. The frequencies of the two genotypes were 38.0% and 62.0% in the BPD group, 29.0% and 71.0% in the control group. The C allele frequencies were 31.0% in the BPD group and 35.5% in the control group. The T allele frequencies were 69.0% in the BPD group and 64.5% in the control group. No significant differences existed in allele and genotype frequencies between the two groups （P=0.178 and 0.338）. The frequencies of the two genotypes in the Mongolian BPD infants and the Han BPD infants were 40.0% and 60.0% vs. 36.0% and 64.0%. The C allele frequencies were 30.0% and 32.0%, and the T allele frequencies were 70.0% and 68.0%. No significant differences existed in allele and genotype frequencies between the two groups （P=0.680 and 0.648）.ConclusionNo correlation existed between the polymorphism of VEGF-460T〉C locus and the occurrence and development of BPD in premature infants in Inner Mongolia

关 键 词：支气管肺发育不良 血管内皮生长因子类 基因测定 多态性 单核苷酸 婴儿 早产 

分 类 号：R722.6[医药卫生—儿科]