产焦性没食子酸菌株筛选及其培养条件优化  

Screening and Optimization of Culture Condition of a Pyrogallol-producing Strain

作　　者：徐曼[1,2,3,4] 汪咏梅 张亮亮[1,2,3,4] XU Man;WANG Yongmei;ZHANG Liangliang(Institute of Chemical Industry of Forest Products,CA;National Engineering Lab.for Biomass Chemical Utilizatio;Key and Open Lab.of Forest Chemical Engineering,SF;Key Lab.of Biomass Energy and Material,Jiangsu Province,Nanjing 210042,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042

出　　处：《林产化学与工业》2018年第3期97-102,共6页Chemistry and Industry of Forest Products

基　　金：国家863计划资助项目(SS2014AA021802)

摘　　要：研究了肠杆菌、短乳杆菌和植物乳杆菌3种菌种催化底物没食子酸降解产焦性没食子酸的能力,其中产焦性没食子酸效果较好的是肠杆菌和植物乳杆菌。对不同温度、底物浓度、pH值、离子浓度等反应条件对肠杆菌和植物乳杆菌没食子酸脱羧酶酶活的影响进行分析,并确定了这两株菌降解没食子酸的培养条件。结果表明:在培养温度为28℃、没食子酸浓度为30 mmol/L,(NH_4)_2SO_4浓度8 mmol/L,MgSO_4浓度为80 mmol/L,初始培养基pH值6.0,培养48 h时,肠杆菌催化没食子酸产焦性没食子酸58.11%;在培养温度为37℃、底物浓度为10 mmol/L,(NH_4)_2SO_4浓度为8 mmol/L,MgSO_4浓度为20 mmol/L,初始培养基pH值6.5,培养48 h时,植物乳杆菌催化没食子酸产焦性没食子酸83.31%。Three bacteria strains including Enterobacter spp.,Lactobacillus brevis and Lactobacillus plantarum were tested for degrading gallic acid into pyrogallol. The factors affecting the activity of gallate decarboxylase from E. spp. and L. plantarum,including substrate concentration, temperature, pH, magnesium sulfate（ MgSO4） concentration were also studied. The experiment results showed that the yield of pyrogallol by E. spp. could reach 58. 11% with the temperature 28 ℃,30 mmol/L gallic acid,8 mmol/L（ NH4）2SO4,80 mmol/L MgSO4 and pH 6.0 after cultured for 48 h. Meanwhile,the yield of pyrogallol by L. plantarum could reach 83.31% with the temperature 37 ℃,substrate concentration 10 mmol/L,concentration of（ NH4）2SO48 mmol/L,concentration of MgSO420 mmol/L and pH 6.5 after cultured for 48 h.

关 键 词：没食子酸 焦性没食子酸 没食子酸脱羧酶 肠杆菌 植物乳杆菌 

分 类 号：TQ35[化学工程]