长链非编码RNA MALAT-1基因的敲除抑制人喉鳞状细胞癌Hep-2细胞的增殖和迁移  

Long chain non-coding RNA MALAT-1 gene knockdown inhibits growth and migration and promotes apoptosis of human laryngeal squamous cell carcinoma Hep-2 cells in vitro

作　　者：韩跃峰[1] 陈德尚[1] 李慧[1] 王晓敏[1] 张明洁[1] 杨洋 HAN Yuefeng;CHEN Deshang;LI Hui;WANG Xiaomin;ZHANG Mingjie;YANG Yang(Department of Otolaryngology,Head and Neck surgery,First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,Chin)

机构地区：[1]蚌埠医学院第一附属医院耳鼻喉头颈外科,安徽蚌埠233004

出　　处：《南方医科大学学报》2018年第8期923-930,共8页Journal of Southern Medical University

基　　金：安徽省教育厅重点项目(KJ2015A284)

摘　　要：目的探讨敲除长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞的影响。方法使用实时聚合酶链反应(RTPCR)以永生化鼻咽上皮(NPE)细胞株NP-69作为参照检测Fa Du、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达,使用shmalat1慢病毒转染Hep-2细胞,RT-PCR检测其MALAT1的表达。增殖速率分析,MTT法明确MALAT-1对细胞增殖的影响。流式细胞术分析细胞周期和细胞凋亡。采用Transwell小室检测Hep-2细胞的迁移。最后使用Matrigel侵袭实验评估shmalat1和sh Ctrl慢病毒转染的Hep-2细胞的侵袭能力。结果使用qPCR检测Fa Du、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达,结果发现与NP-69细胞相比,Fa Du、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达显著增加。敲除MALAT1明显抑制Hep-2细胞增殖。与MOCK和sh Ctrl细胞相比,MALAT1-sh RNA慢病毒转染细胞S期细胞数量显著增加(P<0.01)。此外,细胞在G2/M期的百分比下降(P<0.01)。下调MALAT1时,Hep-2细胞的侵袭和迁移都受到抑制。结论 MALAT1在喉鳞状细胞癌高表达。敲除长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞的增殖、凋亡、侵袭和迁移起抑制作用。Objective To investigate the effect of knocking down long chain non-coding RNA MALAT-1 gene on the biological behaviors of human laryngeal squamous cell carcinoma Hep-2 cells. Method With immortalized nasopharyngeal epithelial（NPE） cell line NP-69 as the reference, MALAT1 expression in Fa Du, Hep-2 and nasopharyngeal carcinoma CNE-2 Z cells were detected using real-time PCR. Hep-2 cells were transfected with shmalat1 lentivirus and the expression of MALAT1 was detected. MTT assay, flow cytometry, Transwell assay and M Atrigel invasiveness test were used to evaluate the effect of MALAT-1 knockdown on the proliferation, cell cycle, cell apoptosis, migration, and invasiveness of Hep-2 cells. Results Compared with NP-69 cells, Hep-2 cells, Fa Du cells, and CNE-2 Z cells all showed significantly increased MALAT-1 expression. In Hep-2 cells, knockdown of MALAT-1 significantly inhibited the cell proliferation, increased the cell percentage in S phase（P〈0.01）, decreased the cell percentage in G2/M phase（P〈0.01）, and attenuated the migration and invasiveness of the cells. Conclusion MALAT-1 is over-expressed in laryngeal squamous cell carcinoma, and knocking down MALAT-1 gene can significantly suppress the proliferation, invasion and migration and promotes apoptosis of the cancer cells.

关 键 词：细胞凋亡 长链非编码RNA 喉肿瘤 MALAT1 迁移和侵袭 

分 类 号：R739.65[医药卫生—肿瘤]