IL-9在结肠癌组织中的表达及其临床意义  

Expression of interleukin-9 in colon cancer tissues and its clinical significance

作　　者：王进[1] 董晓强[1] 朱新国[1] 赵华[1] 毛德利 赵鑫[1] WANG Jin;DONG Xiaoqiang;ZHU Xinguo;ZHAO Hua;MAO Deli;ZHAO Xin(Department of General Surgery,First Affiliated Hospital of Soochow University,Suzhou 215006,China)

机构地区：[1]苏州大学附属第一医院普外科,江苏苏州215006

出　　处：《南方医科大学学报》2018年第8期943-948,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(31770985;81302146);中国博士后基金(2016M591913);江苏省自然科学基金(BK20161225);江苏省卫生计生委科研课题(H201620);苏州市科技计划(SYS201539);江苏省青年医学人才(QNRC2016732);江苏省"六大人才高峰"计划资助项目(2016-WSW-043)~~

摘　　要：目的探讨IL-9在结肠癌组织中的表达水平及其临床意义。方法采用免疫组化法和实时荧光定量PCR(q RT-PCR)法分别检测92例结肠癌组织及相应癌旁正常组织中IL-9的表达情况;统计分析IL-9在结肠癌与癌旁正常组织中的表达差异;分析IL-9蛋白表达与结肠癌患者的临床病理特征及生存预后之间的关系。结果在癌旁正常组织中IL-9蛋白及m RNA的表达水平均显著高于结肠癌组织(P<0.001)。IL-9蛋白表达水平与结肠癌患者的TNM分期、Ducks分期及淋巴结转移相关(P=0.013,0.025,0.004),而与性别、年龄、肿瘤大小、分化程度及肝转移等临床病理特征无明显相关性(P>0.05)。IL-9表达阳性结肠癌患者的生存时间明显长于阴性表达的患者(P=0.015)。结论 IL-9在结肠癌组织中较在癌旁正常组织中低表达;IL-9在结肠癌组织中的表达与TNM分期、Ducks分期及淋巴结转移呈负相关,与良好预后呈正相关,提示在结肠癌肿瘤微环境中IL-9发挥了重要的作用。Objective To investigate the expression of interleukin-9（IL-9） in colon cancer tissues and its clinical significance.Methods Immunohistochenmistry and q RT-PCR were used to detect the expressions of IL-9 protein and m RNA in 92 colon cancer tissues and paired adjacent normal tissues. The correlation of IL-9 expressions with the clinicopathological features and prognosis of the patients was analyzed. Results IL-9 protein and m RNA expressions were significantly higher in adjacent normal tissues than in the colon cancer tissues（P〈0.001）. In colon cancer patients, IL-9 expression was significantly correlated with TNM stage（P=0.013）, Ducks stage（P=0.025） and lymph node metastasis（P=0.004） but not with gender, age, tumor size,differentiation or hepatic metastasis（P〉0.05）. The survival time of colon cancer patients with positive IL-9 expression was significantly longer than that of patients negative for IL-9 expression（P=0.015）. Conclusions IL-9 expression is lowered in colon cancer tissues compoved with in the adjacent normal tissues. IL-9 expression is negatively correlated with TNM staging,Ducks staging and lymph node metastasis but positively with good prognosis, suggesting its important role in the tumor microenvironment of colon cancer.

关 键 词：IL-9 结肠癌 免疫组化 预后 

分 类 号：R735.35[医药卫生—肿瘤]