鹿茸多肽蛋白的提取及功能研究  

Study on the extraction and function of Antler Polypeptide Protein

作　　者：高冷[1] 王昊天 牛晓晖 杨富雅 吕思丞 高晓晨[2] GAO Leng;WANG Haotian;NIU Xiaohui;YANG Fuya;LYU Sichengl;GAO Xiaochen(Changchun University of Technology,Changchun 130012,China;Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春工业大学,长春130012 [2]长春中医药大学,长春130117

出　　处：《吉林中医药》2018年第7期825-828,共4页Jilin Journal of Chinese Medicine

基　　金：吉林省教育厅科学技术研究项目(吉教科合字[2016]第29号)

摘　　要：目的研究鹿茸多肽蛋白的提取较佳方法,以及研究其是否有抗感染效果。方法通过超声法和CO_2超临界萃取法提取鹿茸蛋白,对比两种方法提取率,并确定较佳提取方法。之后酶解鹿茸多肽蛋白,NIH3T3细胞实验来研究鹿茸多肽蛋白的抗炎效果。结果 CO_2超临界萃取法的提取率高于超声法,中高剂量的酶解鹿茸多肽蛋白有显著的抑制炎症效果。结论 CO_2超临界萃取法为较佳的提取工艺,且鹿茸多肽蛋白有抗感染效果。Objective To study the better extraction method of antler polypeptide protein and whether it has anti-inflammatory effect. Methods The yield of antler protein was compared by ultrasonic extraction and CO2 supercritical extraction. Afterwards, the anti-inflammation effect of antler polypeptide protein was studied by enzymatic hydrolysis of antler polypeptide protein and NIH3T3 cell experiment. Results The extraction rate of CO2 supercritical extraction is higher than that of ultrasonic extraction, and the medium and high dose enzymatic hydrolysis of antler polypeptide protein has a significant anti-inflammatory effect. Conclusion CO2 supercritical extraction is a better extraction process, and antler polypetide Protein has anti-inflammatorv effect.

关 键 词：鹿茸蛋白 提取 抗感染 CO2超临界萃取法 

分 类 号：R284.2[医药卫生—中药学]