PIK3CA及GST-π在人骨肉瘤中的表达及其临床意义  

Expression of PIK3CA and GST-π in human osteosarcoma and its clinical significance

作　　者：丁万军[1] 黄莉 曾涛[3] 戈伟[1] 张煜坤[1] 郭卫春[4] 余铃[4] DING Wanjun;HUANG Li;ZENG Tao;GE Wei;ZHANG Yukun;GUO Weichun;YU Ling(Department of Oncology,Renmin Hospital of Wuhan University,Hubei Province,Wuhan 430060,China;Department of Oncology,Panjiang Investment Holding（Group）Limited Company General Hospital,Guizhou Province,Liupanshui 561609,China;Department of Endocrinology,East Campus of Renmin Hospital of Wuhan University,HubeiProvince,Wuhan 430000,China;Department of Orthopedics,Renmin Hospital of Wuhan University,HubeiProvince,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院肿瘤中心,湖北武汉430060 [2]贵州盘江投资控股(集团)有限公司总医院肿瘤科,贵州六盘水561609 [3]武汉大学人民医院东院内分泌科,湖北武汉430000 [4]武汉大学人民医院骨科,湖北武汉430060

出　　处：《中国医药导报》2018年第16期21-24,182,共5页China Medical Herald

基　　金：武汉大学青年教师资助项目(2042014kf0182)

摘　　要：目的检测磷脂酰肌醇3激酶(PIK3CA)及谷胱甘肽-S-转移酶π(GST-π)在骨肉瘤组织中的表达,探讨其对骨肉瘤多药耐药的影响。方法收集武汉大学人民医院2009年1月~2017年6月33例骨肉瘤病理组织标本,应用免疫组化方法(Envision法)及Western blot法测定PIK3CA、GST-π在骨肉瘤标本中的表达。比较化疗耐药组(7例)和化疗有效组(26例)中表达的差异,并结合临床病理因素进行分析。结果免疫组化检测化疗耐药组和化疗有效组的PIK3CA表达的平均吸光度值分别为(11.089±2.147)、(6.938±2.065),差异有统计学意义(P<0.05);化疗耐药组和化疗有效组GST-π表达的平均吸光度值分别为(15.871±2.678)、(7.693±2.443),差异有统计学意义(P<0.05)。Western blot法检测两组中PIK3CA和GST-π的蛋白表达,差异均有统计学意义(P<0.05)。PIK3CA和GST-π在骨肉瘤组织中的蛋白表达具有相关性(r=0.68,P<0.01)。结论 PIK3CA及GST-π在骨肉瘤化疗耐药组中高表达,且两者表达呈正相关,其可能是骨肉瘤耐药的发生机制之一。Objective To detect the expression of phosphatidylinositol 3-kinase（PIK3 CA） and glutathione-S transferase-π（GST-π） in osteosarcoma tissues and to explore its effect on the multidrug resistance of osteosarcoma.Methods Thirty-three cases of pathological tissue specimens of osteosarcoma from Renmin Hospital of Wuhan University from January 2009 to June 2017 were collected.The expression of PIK3 CA and GST-π of 33 cases of osteosarcoma specimens was detected by immunohistochemistry（Envision method） and Western blot method.The differences between the chemotherapy-resistant group（7 cases） and the chemotherapy-effective group（26 cases） were compared.The results were analyzed combined with clinicopathological factors.Results The average optical density of PIK3 CA in the chemotherapy-resistant group and chemotherapy-effective group was（11.089±2.147） and（6.938±2.065） respectively,and there was a significant difference between the two groups（P〈0.05）;the average optical density of GST-π in the chemotherapy-resistant group and chemotherapy-effective group was（15.871±2.678） and（7.693±2.443）,and there was a significant difference between the two groups（P 0.05）.The average optical density of PIK3 CA and GST-π by Western blot method had significant differences between the two groups（P 0.05）.The expression of PIK3 CA and GST-π in osteosarcoma was correlated（r = 0.68,P 0.01）.Conclusion PIK3 CA and GST-π are over expressed in osteosarcoma chemotherapy-resistant group,and the expression of PIK3 CA and GST-π is positively correlated with each other,which may be one of the mechanisms of drug resistance in osteosarcoma.

关 键 词：骨肉瘤 磷脂酰肌醇3激酶 谷胱甘肽转移酶 多药耐药 

分 类 号：R73[医药卫生—肿瘤]