翠竹花粉的活力及储藏效果研究  

Germination and Storage Characteristics of Pleioblastus pygmaeus Pollen

作　　者：范婷婷[1,2] 郭臻昊 傅华君 张莉[1,2] 林树燕[1,2] 丁雨龙[1,2] FAN Ting-ting1,2,GUO Zhen-hao1,2,FU Hua-jun1,2,ZHANG Li1,2,LIN Shu-yan1,2,DING Yu-long1,2(1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037, Jiangsu, China;2. Research Institute of Bamboo, Nanjing Forestry University, Nanjing 210037, Jiangsu, Chin)

机构地区：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037 [2]南京林业大学竹类研究所,江苏南京210037

出　　处：《林业科学研究》2018年第3期180-185,共6页Forest Research

基　　金："十二五"国家科技支撑计划项目(2015BAD04B0305);"十三五"国家重点研发计划课题(2016YFD0600901);江苏省三新工程项目(LYSX[2016]04);江苏省科技支撑计划(农业)重点项目(BE2016304)

摘　　要：[目的]以南京林业大学竹种园内开花的翠竹花粉为试验材料,研究在不同条件下花粉的活力和储藏效果,为翠竹杂交育种的生产实践提供基础理论数据。[方法]利用花粉离体培养法研究花粉在不同培养基组分、不同采样时间、不同温度及储藏条件下的萌发特性。[结果]翠竹花粉在不同浓度配比的培养基中萌发率差异显著,以10%蔗糖+0.01%H3BO3+0.06%CaCl_2·2H_2O培养基上培养2.5 h花粉萌发率最高;一天之中以上午9:00的花粉萌发率最高;翠竹花粉离体萌发适合的温度范围较宽,在26 30℃内花粉都有较高的萌发率。随着储藏时间的延长,翠竹花粉萌发率逐渐下降;不同储藏方式对花粉的萌发率有较大的影响,在储藏过程中未经干燥处理的花药的花粉萌发效果好于干燥后的,4℃低温保存下花粉萌发率相对较好,可延长花粉的储藏寿命,96.0 h后仍保有活力。[结论]翠竹花粉在10%蔗糖、0.01%H3BO3、0.06%CaCl_2·2H_2O的培养基中萌发效果最好,萌发适宜的温度范围是2630℃,在储藏过程中低温不干燥的环境有利于延长花粉寿命。[Objective] The flowering Pleioblastus pygmaeus in the bamboo garden of Nanjing Forestry University was used as study object to study the viability and storage of the bamboo pollen under different conditions,and provide data for the production practice of hybrid breeding. [Method] The effects of culture medium composition,sampling time,culture time and preservation conditions on pollen germination of P. pygmaeus were investigated with liquid culture. [Result] The pollen germinating percentage varied with different culture media and reached the highest at the medium with 10% source ＋ 0. 01% H_3BO_3＋ 0. 06% CaCl_2·2 H_2O for 2. 5 h; the suitable temperature range for pollen germination was at 26 30℃. The pollen germinated at a decreasing rate as their storage prolonged. While preserving the pollen,the pollen germination of the fresh anthers was better than that after drying,and that the longevity of pollen could be extended appropriately at 4℃,in which the vitality of pollen maintained for96. 0 h. [Conclusion]The pollen of P. pygmaeus is suitable to cultivate in the culture medium with 10% source ＋0. 01% H_3BO_3＋ 0. 06% CaCl_2·2 H_2O. The suitable cultivation temperature is 26 30℃,and the suitable conservation condition is low temperature and non-drying environments which help prolong the life of pollen.

关 键 词：翠竹 花粉萌发 温度 储藏方式 

分 类 号：S722[农业科学—林木遗传育种]