基于改进MILP算法的甘油发酵产1,3-丙二醇最优可行代谢路径分析  

Analysis on Optimal Feasible Metabolic Pathway of 1,3-Propanediol Fermented from Glycerol Based on an Improved MILP Algorithm

作　　者：禹超[1] 郭通[1] 李永磊[1] 张青瑞[1] YU Chao;GUO Tong;LI Yong-lei;ZHANG Qing-rui(College of Chemical Engineering,Qingdao University o f Science and Technology,Qingdao 26600.2,Ghin)

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《化学与生物工程》2018年第6期21-24,共4页Chemistry & Bioengineering

基　　金：山东省自然科学基金项目(ZR2013BM001)

摘　　要：针对混合整数线性规划(MILP)算法求解所有最优可行解时变量搜索范围过大的问题,提出了用通量可变性分析(FVA)改进MILP算法;并以克雷伯氏杆菌发酵甘油产1,3-丙二醇(1,3-PD)为研究对象,以1,3-PD产率最大为优化目标,利用改进MILP算法得到不同氧气消耗下所有最优代谢路径;进一步通过ATP消耗总量分析,确定ATP消耗最小的最优代谢路径。To solve the problem that the searching scope for variables is too large while solving all optimalfeasible solutions by the mixed integer linear programming（MILP ） algorithm,we proposed an improved MILP algorithm incorporating the flux variability analysis（FVA ）. Using 1,3-propanediol（1,3-PD ） fermentated from glycerol by Klebsiella pneumoniae as a research subject,and the maximum yield of 1 ,3-PD as an optimized tar-get, we obtained all optimal metabolic pathways under different oxygen consumption by the improved M I L P al-gorithm. In addition,we determined the optimal metabolic pathway with the minimum ATP total consumption through analyzing ATP total consumption.

关 键 词：混合整数线性规划(MILP)通量可变性分析(FVA)克雷伯氏杆菌 发酵 l 3-丙二醇 最优代谢路径 

分 类 号：TQ923[轻工技术与工程—发酵工程]