肝癌易感性与端粒酶逆转录酶基因多态性的相关性研究  

Association of genetic variants of hTERT with susceptibility to hepatocellular carcinoma

作　　者：崔伟丽 褚志予 马红[2] 崔京远[3] 亓玉琴[4] CUI Wei-li;CHU Zhi-yu;MA Hong;CUI Jing-yuan;Qi Yu-qin(Department of Liver Disease,QingDao No.6 People’s Hospital（Qingdao 266033,China;Department of Rheumatism Immunity;Department of General Surgery,4Department of Gastroenterology,QingDao Municipal Hospital（Qingdao 266011,China)

机构地区：[1]青岛市第六人民医院肝病科,山东青岛266033 [2]青岛市市立医院风湿免疫科,山东青岛266011 [3]青岛市市立医院普外科,山东青岛266011 [4]青岛市市立医院消化内一科,山东青岛266011

出　　处：《中国现代普通外科进展》2018年第4期253-256,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨人类端粒酶逆转录酶(h TERT)基因位点(rs 2736100、rs 2853676)单核苷酸多态性(SNP)与青岛地区人群乙肝肝癌的易感性。方法:采用聚合酶链反应-限制性片段长度多态性(PCRRFLP)检测来自青岛地区的肝癌患者165例、非肝癌乙肝病毒感染者195例、健康对照者402例h TERT基因型分布。结果:(1)肝癌组患者rs2736100位点GG基因型频率显著高于对照组、非肝癌HBV感染者及所有非HCC者(均P<0.05),携带GG基因型者罹患肝癌的风险分别增加至2.13倍、2.17倍和2.14倍。(2)肝癌组患者rs 2853676位AA基因型频率显著高于对照组、非肝癌HBV感染者及所有非HCC者(均P<0.05),携带AA基因型者罹患肝癌的风险分别增加至3.42倍、2.39倍和3.02倍。结论 :h TERT rs 2736100和rs 2853676位点基因多态性与肝癌易感性相关。Objective： To explore the correlation between several single-nucleotide polymorphisms（SNPs） loci on the gene of human telomerase reverse transcriptase（hTERT）（including rs2736100、rs2853676） and the susceptibility to Hepatocellular carcinoma in Qingdao area. Meth-ods： This case-control study included 165 diagnosed Hepatocellular carcinoma, 195 with chronic hepatitis B and 402 Healthy people. Polymerase chain restriction fragment length polymorphism were performed to analyze the genotype of the loci on the gene of hTERT. Results： The frequency of TT genotype on rs2736100 were higher in the Hepatocellular carcinoma group compared to the control group、chronic hepatitis B and no HCC people（P〈0.05）. The additive odds ratio of TT genotype for the risk of Hepatocellular carcinoma was 2.13、2.17 and 2.14. The frequency of AA genotype on rs2853676 were higher in the Hepatocellular carcinoma group compared to the control group、chronic hepatitis B and no HCC people（P〈0.05）. The additive odds ratio of TT genotype for the risk of Hepatocellular carcinoma was 3.42、2.39 and 3.02. Conclusion： There was a relationship between hTERT rs2736100 and rs2853676 polymorphism and susceptibility to Hepatocellular carcinoma.

关 键 词：肝癌 端粒 末端转移酶 单核苷酸多态性 HBV感染 

分 类 号：R735.7[医药卫生—肿瘤]