血清CD146表达在结直肠癌早期诊断中的价值及机制  

The value and mechanism of CD146 expression in the early diagnosis of colorectal cancer

作　　者：邱芳华[1] 刘道利[2] 龙华婧[2] 杨泽填 刘丽芳[3] QIU Fanghua;LIU Daoli;LONG Huajing;YANG Zetian;LIU Li fang(Department of Infectious Disease;Department of Clinical Laboratory,Guangzhou Hospital of Traditional Chinese Medicine,Guangzhou,Guangdong 510130,China;Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510130,China)

机构地区：[1]广州市中医医院感染科,510130 [2]广州市中医医院检验科,510130 [3]广州中医药大学,510130

出　　处：《检验医学与临床》2018年第12期1697-1700,共4页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81503424);广东省自然科学基金资助项目(2014A030313802);广东省广州市产学研协同创新重大专项资助项目(201704020171)

摘　　要：目的明确血清CD146在结直肠癌(CRC)早期筛查中的诊断价值及机制。方法收集22例新鲜结直肠癌患者的标本(癌及对应的癌旁组织各11例),对YAP及CD146进行免疫印迹法(WB)检测;在结直肠癌细胞株HCT-116及HT-29中过表达YAP后采用WB检测CD146表达;选取健康对照者40例作为对照组,腺瘤患者40例作为腺瘤组,以及167例结直肠癌患者作为CRC组,采用酶联免疫吸附试验检测血清CD146水平。结果 CD146及其调控因子YAP在结直肠癌组织中明显表达上调,HCT-116及HT-29细胞中过表达YAP可明显上调CD146表达;CRC组血清CD146水平明显高于对照组和腺瘤组,差异均有统计学意义(P<0.05);CRC患者切除术后血清CD146明显低于术前。血清CD146水平与结直肠癌分化、肿瘤细胞浸润程度、远处转移呈正相关。结论血清CD146有望作为CRC诊断及预后判断的分子标志物,CD146的表达与YAP密切相关。Objective To clarify the diagnostic value and mechanism of serum CD146 in early screening of the colorectal cancer.Methods A total of 22 tissue samples of colorectal cancer patients were collected for WB detection of YAP and CD146;CD146 expression was detected by WB after overexpression of YAP in HCT116 and HT-29 of colon cancer cell lines.A total of 40 cases of healthy persons were taken as comtrol group.A total of 40 cases of adenoma patients were taken as adenoma group and 167 cases of colorectal cancer were taken as colorectal cancer group,serum CD146 concentration was detected by ELISA.Results The expressions of CD146 and its regulatory factor YAP were obviously up-regulated in colorectal carcinoma.The overexpression of YAP in HCT-116 and HT-29 cells can obviously lead to the up-regulation of CD146 expression,and the level of CD146 in the colon cancer group was significantly higher than that in the healthy control group and the adenoma group,and the difference were statistically significant（P〈0.05）,and the serum CD146 was significantly lower than that before the resection of colon cancer.Serum CD146 level was positively correlated with intestinal differentiation,tumor cell infiltration and distant metastasis.Conclusion Serum CD146 can be expected to be a molecular marker for the diagnosis and prognosis of colorectal cancer.The expression of CD146 is closely related to YAP.

关 键 词：结直肠癌 CD146 YAP调控 

分 类 号：R735.3[医药卫生—肿瘤]