乳腺癌组织中人乳腺球蛋白的表达水平及其与乳腺癌发生和发展的关系  

Expression of human mammary globulin in breast cancer tissues and its relationship with the occurrence and development of breast cancer

作　　者：王刚 廖翠屏 刘敏 温亚楠 WANG Gang;LIAO Cuiping;LIUMin;WEN Yanan(Department of Clinical Laboratory,Maternal and Child Health Care Hospital District,Chengdu,Sichuan 611130,China)

机构地区：[1]四川省成都市温江区妇幼保健院检验科,611130

出　　处：《检验医学与临床》2018年第12期1789-1791,1795,共4页Laboratory Medicine and Clinic

摘　　要：目的研究乳腺癌组织中人乳腺球蛋白的表达水平及其与乳腺癌发生和发展的关系。方法收集该院2015年1月至2017年8月乳腺癌组织(A组)、癌旁正常组织(B组)及乳腺良性病变组织(C组)标本各40例,通过组织微阵列技术与免疫组化方法对3组中人乳腺球蛋白的表达水平进行测定,并通过反转录聚合酶链反应对3组中人乳腺球蛋白mRNA表达进行测定,比较3组人乳腺球蛋白及其mRNA表达情况。结果 A组人乳腺球蛋白阳性率明显高于B、C组,且其mRNA表达水平也明显高于B、C组,差异均有统计学意义(P<0.05);而B组与C组人乳腺球蛋白阳性率及其mRNA表达水平比较,差异均无统计学意义(P>0.05)。乳腺癌组织中人乳腺球蛋白的表达与组织学分级、淋巴结转移及雌激素受体(ER)密切相关(P<0.05),而与病理类型、TNM分期、肿瘤大小及孕激素受体(PR)均无明显相关性(P>0.05)。多因素Logistic回归分析发现,乳腺癌组织中人乳腺球蛋白的表达与组织学分级、PR及ER水平均存在明显关系(P<0.05)。结论人乳腺球蛋白在乳腺癌组织中呈高表达水平,并且与组织学分级、PR及ER密切相关,可能参与乳腺癌的发病过程,可作为评估患者肿瘤侵袭能力及恶性程度的重要指标。Objective To study the expression of human mammary globulin in breast cancer and its relationship with the occurrence and development of breast cancer.Methods The breast cancer tissues（group A）and adjacent normal tissues（group B）and benign breast lesion tissues（group C）were selected in the hospital,with 40 cases in each group.The the expression levels of human mammary globulin in the three groups were determined by tissue microarray technique and immunohistochemistry,and the human mammary globulin mRNA expression in the three groups was determined by reverse transcription polymerase chain reaction.The expression of human mammary globulin and its mRNA were compared among the three groups.Results The positive rate of human mammary globulin in the group A was significantly higher than that in the group B and group C,and the expression level of mRNA in the group A was significantly higher than those in the group B and group C（P〈0.05）,but there was no significant difference between group B and group C（P〈0.05）.The expression of human mammary globulin in breast cancer tissues was significantly related to histological grade,lymph node metastasis and estrogen receptor（ER）（P〈0.05）,but had no significant correlation with the pathological type,TNM stage,tumor size and progesterone receptor（PR）（P〈0.05）.Multivariate Logistic regression analysis showed that there were significant correlation between the expression of human breast globulin and histological grade,PR and ER（P〈0.05）.Conclusion Human mammary globulin shows high expression levels in breast cancer tissues,and it is significant correlated to the histological grade,PR and ER,indicating that it may be involved in the pathogenesis of the breast cancer,so it can be used as an important index to evaluate the tumor invasion and malignant degree of breast cancer.

关 键 词：乳腺癌 乳腺良性病变 人乳腺球蛋白 

分 类 号：R737.9[医药卫生—肿瘤]