金针菇中棒曲霉素高效液相色谱检测法的研究  

Study on Chromatography for Determination of Patulin in Flammulina velutipes by HPLC

作　　者：石宝俊[1,2] 葛玲 姜洪芳 林群英[1] 朱海亮[3] 张卫明[1] Shi Baojun1,2 , Ge Ling2,Jiang Hongfang1,Lin Qunying1, Zhu Hailiang3, Zhang Weiming1(1. Nanjing Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, China;2. Elionnature Biotech Co. , Ltd. , Nanjing 210038, China; 3. School of Life Sciences,Nanjing University, Nanjing 210093,Chin)

机构地区：[1]南京野生植物综合利用研究院,江苏南京210042 [2]亿利耐雀生物科技有限公司,江苏南京210038 [3]南京大学生命科学学院,江苏南京210093

出　　处：《中国野生植物资源》2018年第1期22-26,共5页Chinese Wild Plant Resources

基　　金：国家科技支撑计划资助项目(2015BAD16B07)

摘　　要：对金针菇中棒曲霉素的分析检测方法进行了研究,旨在建立一种以液-液萃取为前处理方法,采用高效液相色谱(HPLC)分析检测金针菇中棒曲霉素含量的方法。主要研究结果如下:通过组织破碎,制备金针菇汁,采用乙酸乙酯重复萃取,用碳酸钠溶液净化萃取液,制备金针菇供试品。该方法处理和测定棒曲霉素精密度高,检出限低。棒曲霉素标准品的标准曲线为y=112.8x-14.23,R2=0.998 4,标准曲线范围为0.01~25μg/m L。In this study,the method for the determination of patulin in Flammulina velutipes was studied. The aim of this study was to establish a method for the determination of patulin in Flammulina velutipes by high performance liquid chromatography（ HPLC） with liquid-liquid extraction as the pretreatment method. Methods. The main results were as follows： through the organization of broken,the preparation of Flammulina velutipes,the use of ethyl acetate repeated extraction,with sodium carbonate solution purification extract,the preparation of Flammulina for the test. The method of treatment and determination of patulin has high precision and low detection limit. The standard curve for the standard test of the term of the standard is y = 112. 8 x-14. 23,R^2= 0. 998 4,and the standard curve is in the range of0. 01μg/m L to 25μg/m L.

关 键 词：金针菇 高效液相 棒曲霉素 萃取 标准曲线 

分 类 号：S646.75[农业科学—蔬菜学]