GC-MS/SIM法同时测定臭冷杉挥发油中柠檬烯和醋酸龙脑酯含量  

Simultaneous Determation of Limonene and Bornyl Acetate in the Essential Oil from Abies nephrolepis by GC-MS/SIM

作　　者：任恒鑫[1] 吕玉光[1] 宗希明[1] 牟立婷 张舒婷[1] 宋春香[1] 孙长海[1] Ren Hengxin,Lu Yuguang,Zong Ximing,Mu Liting,Zhang Shuting,Song Chunxiang,Sun Changhai(College of Pharmacy,Jiamusi University,Jiamusi 154007,Chin)

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007

出　　处：《中国野生植物资源》2018年第1期41-43,50,共4页Chinese Wild Plant Resources

基　　金：国家级项目培育项目(JMSUJCGP2016-003);佳木斯大学科学技术研究项目(13Z1201563)

摘　　要：目的:建立同时测定臭冷杉挥发油中柠檬烯和醋酸龙脑酯含量的方法。方法:采用气相色谱-质谱联用(GC-MS)技术,选择离子模式(SIM)检测,色谱柱为HP-5MS石英毛细管柱(30 m×0.25 mm×0.25μm),初始温度40℃,4℃·min-1升至260℃。载气为氦气。选择m/z为68和95的特征离子分别对柠檬烯和醋酸龙脑酯进行定量。结果:柠檬烯和醋酸龙脑酯在400~4 000μg·m L-1和800~8 000μg·m L-1浓度范围内与峰面积呈良好的线性关系,其相关系数为0.999 8和0.999 6;平均加样回收率为98.5%和98.7%;RSD值为1.3%和1.5%(n=6)。结论:该方法简便、快速,结果准确,可用于臭冷杉挥发油的质量控制。Objective： To develop a new method for the determination of limonene and bornyl acetate content in the essential oil from Abies nephrolepis. Method： The limonene and bornyl acetate were analyzed by gas chromatography-mass spectrometry（ GC-MS）/SIM on the HP-5 MS quartz capillary chromatographic column（ 30 m × 0. 25 mm × 0. 25 μm） and quantified by selective ion monitoring（ SIM）. Initial temperature was set at 40 ℃. It was programmed rising by 4 ℃ ·min-1 until 260 ℃. The selected ions were m/z 68 and 95. Results： A good linear relationship was achieved for limonene and bornyl acetate over the range of 400-4 000 μg ·m L^-1（ r =0.999 8） and 800-8 000 μg ·m L^-1（ r =0.999 6）. The average recovery was 98. 5%（ RSD = 1. 3 %,n = 6） and 98. 57%（ RSD = 1. 5 %,n = 6）. Conclusion： The method is simple,rapid and accurate,and suitable for the quality control of the essential oil from Abies nephrolepis.

关 键 词：气相色谱-质谱联用技术 选择离子模式 臭冷杉 挥发油 柠檬烯 醋酸龙脑酯 

分 类 号：R284.1[医药卫生—中药学]