TP53基因在维吾尔族食管癌中的基因表型、蛋白表达及其与预后的相关性  

The gene phenotype, protein expression of TP53 gene in Uygur′s esophageal carcinoma and its correlation with prognosis

作　　者：阿不都沙拉木.阿不都热合曼 买地尼也提.尼亚孜 张海平[1] 居来提.艾尼瓦尔 阿不都艾尼.吐尔洪 张力为[1] 伊力亚尔.夏合丁 伊地力斯.阿吾提 Abudoushalamu·Abudoureheman;Madinyet·Niyaz;ZHANG Haiping;Julaiti·Ainiwaer;Abudouaini·Tuerhong;ZHANG Liwei;Ilyar·Shayhedin;Idiris·Awut(Department of Thoracic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830054,China;Institute of Clinical Medicine,the First Affiliated Hospital of XinjiangMedical University,Xinjiang Uygur Autonomous Region,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院胸外科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院临床医学研究院,新疆乌鲁木齐830054

出　　处：《中国医药导报》2018年第17期17-20,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81360305)

摘　　要：目的探讨维吾尔族食管癌组织中TP53基因缺失和p53蛋白表达状况及其与预后的相关性。方法选取2009年12月~2013年6月在新疆医科大学第一附属医院胸外科行根治性手术切除的维吾尔族食管鳞状细胞癌患者67例,应用荧光原位杂交技术检测其间期细胞核中TP53基因缺失情况,并通过免疫组化方法检测p53蛋白表达情况,分析TP53基因表型、蛋白表达与临床病理因素及术后生存期的关系。结果在食管癌高分化组中TP53基因的缺失率为15.00%,而在中、低分化组中为40.43%,差异有统计学意义(P<0.05);而TP53基因缺失在不同肿瘤浸润深度、不同淋巴结转移状态样本中的差异无统计学意义(P>0.05)。p53蛋白表达在不同肿瘤分化程度、不同肿瘤浸润深度和不同淋巴结转移状态样本中的差异均无统计学意义(P>0.05)。结论 TP53基因缺失与食管癌肿瘤分化程度有一定的相关性,但与术后生存期关系不能确定,TP53基因缺失和p53蛋白表达尚不能作为评估维吾尔族食管癌患者预后的生物标志物,需要增加样本量进一步确定。Objective To study the TP53 gene deletion and p53 protein expression level in Uygur′s esophageal carcinoma, and its correlation with prognosis of Uygur esophageal cancer patients. Methods Sixty-seven cases of Uygur esophageal squamous cell carcinoma treated by radical resection from December 2009 to June 2013 in Department of Thoracic Surgery, the First Affiliated Hospital of Xinjiang Medical University were selected. TP53 gene deletion and p53 protein expression was detected respectively by fluorescence in situ hybridization and immuno-histo-chemistry methods. The relationship of TP53 gene deletion and p53 protein expression with clinicopathological factors and postoperative survival rate was analyzed. Results In the highly differentiated group, the deletion rate of TP53 gene was15.00%, which of moderate and poorly differentiation group was 40.43%, the difference was statistically significant（P〈0.05）. There was no significant difference of the deletion rate of TP53 gene in different tumor invasion depth and lymph node metastasis status（P〈0.05）. There was no significant difference of the degree of p53 protein expression in different degrees of tumor differentiation, tumor invasion depth and lymph node metastatis status（ P〉0.05）. Conclusion There is a certain correlation between TP53 gene deletion and the differentiation degree of esophageal carcinoma tumor,but the relationship with the survival has not been determined. The TP53 gene deletion and p53 protein expression level cannot be seen as the prognostic biomarkers of Uygur patients with esophageal cancer. Therefore,larger sample capacity is needed for in-depth exploration and determination.

关 键 词：维吾尔族 食管鳞状细胞癌 P53 预后 

分 类 号：R739.45[医药卫生—肿瘤]