肝细胞肝癌患者外周血CD8^+T淋巴细胞凋亡相关机制探讨  

The related mechanism of CD8^+T lymphocyte apoptosis in patients with hepatocellular carcinoma

作　　者：郭存丽[1] 毕玉美[1] 刘钊[1] 徐易[1] 孙一欣[1] 王秋程[1] Guo Cunli;Bi Yumei;Liu Zhao;Xu Yi;Sun Yixin;Wang Qiucheng(Department of Ultrasound,Caneer Hospital Affiliated to Harbin Medical University,Heilongjiang Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属肿瘤医院超声科,黑龙江哈尔滨150081

出　　处：《现代肿瘤医学》2018年第14期2151-2155,共5页Journal of Modern Oncology

基　　金：国家自然科学基金青年科学基金项目(编号:81501479);黑龙江省卫生计生委科研课题面上项目(编号:2014-348);哈尔滨医科大学附属肿瘤医院海燕基金(编号:JJQN2014-10)

摘　　要：目的:探讨肝细胞肝癌患者外周血CD8^+T淋巴细胞(CTL细胞)凋亡的相关机制及临床意义。方法:收集30例健康体检者及60例不同临床分期肝细胞肝癌患者外周血,应用流式细胞仪检测分析CTL细胞相对百分比、凋亡情况及CTL细胞表面Fas(CD95)表达情况。结果:肝细胞肝癌患者外周血CTL细胞相对百分比含量高于健康体检者[(26.4%±9.2%)vs(19.7%±4.7%),P<0.05]。肝细胞肝癌患者外周血Annexin V+CTL细胞凋亡率高于健康体检者[(25.3%±6.5%)vs(12.1%±6.5%),P<0.05]。肝细胞肝癌患者Fas+CTL细胞含量高于健康体检者[(62.2%±18.5%)vs(42.6%±16.5%),P<0.05]。Fas表达与CTL细胞的凋亡呈正相关。不同TNM分期患者CTL细胞凋亡能力不同,随着病情程度恶化,细胞凋亡水平增加[(27.7%±5.9%)vs(20.6%±5.1%),P<0.001]。结论:肝细胞肝癌患者外周血中CTL细胞的百分比增加,提示在肿瘤的发生发展过程中机体存在抗肿瘤免疫效应,但是基于Fas/FasL途径的CTL细胞凋亡比例增加,可能是肿瘤细胞免疫逃逸的重要机制。Objective： To discuss the related mechanism of CTL lymphocyte apoptosis in patients with hepatocellular carcinoma（ HCC）. Methods： Using flow cytometry to detect peripheral blood CTL lymphocytes percentage,apoptosis,CTL cell surface expression of Fas in the 30 cases of normal people,60 cases of hepatocellular carcinoma patients,analyze their correlation. Results： The hepatocellular carcinoma patients peripheral blood CTL lymphocyte percentage is higher than the normal（ P〈0. 05）. Hepatocellular carcinoma patients peripheral blood Annexin V＋CTL lymphocytes was higher than the normal（ P〈0. 05）. The content of Fas＋CTL cells in patients with liver cancer was higher than that of normal people（ P〈0. 05）. The CTL lymphocyte apoptosis were positively correlated with Fas expression. The apoptosis of CTL cells was different in patients with different stages of TNM. With the deterioration of the condition,the apoptotic level increased（ P〈0. 001）. Conclusion： The percentage of CTL lymphocytes in peripheral blood is increased of patients with hepatocellular carcinoma（ HCC）. But based on Fas/Fas L way of CTL lymphocyte apoptosis percentage increase may be the important mechanism of the immune escape of tumor cells.

关 键 词：肝细胞肝癌 CTL细胞 免疫逃逸 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]