枯草芽胞杆菌表达牛分支杆菌Mtb8.4抗原摇瓶发酵条件优化  

Fermentation Optimization of Bacillus subtilis Expressing rBovMtb8.4

作　　者：李海涛[1,2] 张斌 刘艳环 朱言柱[1,2] 谷晨晨[1] 闫喜军 苗利光 LI Hai-tao;ZHANG Bin;LIU Yan-huan;ZHU Yan-zhu;GU Chen-chen;YAN Xi-jun;MIAO Li-guang(Institute of Special Wild Economic Animal and Plants,CAAS,Changchun,Jilin,130112,China;Key Laboratory of Special Animal Epidemic Disease,Ministry of Agriculture,Changchun,Jilin,130112,China;Veterinary Biological Products Supply Station,Shijiazhuang,Hebei,050000,China)

机构地区：[1]中国农业科学院特产研究所,吉林长春130112 [2]农业部经济动物疫病重点实验室,吉林长春130112 [3]石家庄市兽用生物制品供应站,河北石家庄050000

出　　处：《动物医学进展》2018年第6期63-65,共3页Progress In Veterinary Medicine

摘　　要：为了建立枯草芽胞杆菌摇瓶发酵表达牛分支杆菌Mtb8.4抗原的制备方法,对已构建的含有pNC-HisE-Mtb8.4质粒的分泌表达载体,在枯草杆菌表达系统中进行分泌性表达,采用单因素影响试验方法,在摇瓶水平上进行了表达产量优化。结果表明,各因素的最佳条件是2SY培养基、接种浓度为6%、18h最佳种子阶段,在33℃的培养温度下振荡培养48h,目的蛋白的表达量能够达到750mg/L。该表达条件的确立,为后期生产工艺放大奠定了基础。In order to establish preparation method of Bacillus subtilis fermentation expression of Mycobacterium bovis Mtb8.4 antigen,the constructed plasmid containing pNC-HisE-Mtb8.4 expression vectors was used to express in the system of secretory expression of Bacillus subtilis.The expression yield was optimized in shake flask level by the single factor influence test.The results showed that the optimum conditions for each factor were 2 SY medium,inoculation concentration of 6%and 18 hoptimum seed stage,shaking culture 48 hat 33℃,and the expression of target protein could reach 750 mg/L.The establishment of the expression condition laid the foundation for the enlargement of the later production process.

关 键 词：枯草芽胞杆菌 牛分支杆菌Mtb8.4抗原 发酵 

分 类 号：S852.6[农业科学—基础兽医学]