马尾松树皮提取物对牙本质小管的封闭效果及其耐磨损性能的体外研究  

Sealing effects of pinus massoniana bark extract on dentin tubule and its wear resistance in vitro

作　　者：唐成芳[1] 杨黎燕[2] 曾辉[1] 马新扬[1] 王萌 李经纬[3] TANG Cheng-fang;YANG Li-yan;ZENG Hui;MA Xin-yang;WANG Meng;LI Jing-wei(Department of Stomatology,Xi＇an Medical College,Xi＇an 710021;Department of Pharmacy,Xi＇an Medical College,Xi＇an 710021;Department of Oral and Maxillofacial Surgery,Stomatology Hospital of Xi＇an Jiaotong University,Xi＇an 710004,China)

机构地区：[1]西安医学院口腔医学系,陕西西安710021 [2]西安医学院药学院,陕西西安710021 [3]西安交通大学口腔医院口腔颌面外科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2018年第4期573-577,588,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81641150);陕西省科技厅社会发展项目(No.2015SF242);陕西省教育厅专项科研计划项目(No.16JK1659)~~

摘　　要：目的观察马尾松树皮提取物(PMBE)局部短暂预处理对牙本质小管的即刻封闭效果及耐磨损性,为其防治牙本质过敏提供实验依据。方法用酸蚀法建立牙本质过敏体外模型,分别以乙醇和氟保护漆为阴性对照和阳性对照,采用80g/L PMBE乙醇溶液短暂涂布样本,并进行刷牙磨损,场发射扫描电镜观察各组样本表面和剖面形貌,测量牙本质小管口开放视野面积(ADTO)、牙本质小管口开放相对面积(PADTO)。结果乙醇空白组牙本质表面牙本质小管完全开放,剖面小管腔空虚;氟保护漆/即刻组和PMBE/即刻组牙本质小管完全/基本封闭,管腔内见涂膜或封堵物,氟保护漆/磨损各组及PMBE/磨损各组样本表面见磨痕,牙本质小管部分开口。乙醇空白组ADTO及PADTO显著高于其余各组(P<0.05)。PMBE/磨损各组和氟保护漆/磨损各组ADTO及PADTO组间比较,差异均无统计学意义(P>0.05),但均显著高于PMBE/即刻组和氟保护漆/即刻组(P<0.05)。结论 PMBE局部短暂涂布处理牙本质可获得良好的牙本质小管即刻封闭效果和一定的耐磨性。Objective To observe the sealing effect of transient pinus massoniana bark extract（PMBE）precoating on dentin tubules and its wear resistance so as to provide an experimental basis for PMBE to be used to prevent and treat dentin hypersensitivity.Methods The model of dentin hypersensitivity was established by acid etching method.The samples were transient pre-coating with 80 g/L of PMBE ethanol solution,and then some of them experienced brushing wear treatment with ethanol group and fluorine vanish group as negative and positive controls.The surface and profile morphologies of the samples were observed using field emission scanning electron microscope（FESEM）in each group.The area of dentin tubular openings（ADTO）and percentage area of dentin tubular openings（PADTO）were also measured.Results The dentin tubules were opened completely and the lumen of dentin tubules was empty in ethanol group.The dentin tubules were completely or basically closed and some films or plugging could be seen in the lumen of dentin tubules in fluorine vanish/immediate group and PMBE/immediate group.The dentin tubules were partly opened and some wear marks could be seen on the dentin surface in fluorine vanish/wear groups,PMBE/wear groups.ADTO and PADTO in ethanol group were significantly higher than those in fluorine vanish groups and PMBE groups（P〈0.05）.ADTO and PADTO did not significantly differ between fluorine vanish/wear groups and PMBE/wear groups（P〉0.05）,which were all significantly higher than those in fluorine vanish/immediate group and PMBE/immediate group（P〈0.05）.Conclusion Local transient pre-coating of PMBE ethanol solution can achieve fine immediate sealing effect of dentin tubules and some degree of wear resistance.

关 键 词：牙本质敏感症 马尾松树皮提取物 牙本质小管 耐磨损性 封闭效果 

分 类 号：R782[医药卫生—口腔医学]