抗纤益心方通过TGF-β/Smads信号通路改善糖尿病心肌病大鼠心肌纤维化和心功能的研究  

Influence of Kangxian Yixin prescription on anti-cardiac fibrosis and improvement of cardiac function in diabetic cardiomyopathy rats through transforming growth factor β/Smads signal pathway

作　　者：张会超[1] 聂恒[1] 芮浩淼[1] 曹程浩[1] 杨凤鸣[1] ZHANG Hui-chao,NIE Heng,RUI Hao-miao,CAO Cheng-hao,YANG Feng-ming(Department of Cardiology,Henan Provincial Hospital of TCM, Zhengzhou 450002, Chin)

机构地区：[1]河南省中医院心血管科,河南郑州450002

出　　处：《东南大学学报（医学版）》2018年第3期415-419,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金青年基金资助项目(81403330)

摘　　要：目的:研究抗纤益心方是否通过转化生长因子(TGF)-β/Smads信号通路参与改善糖尿病心肌病(DC)大鼠心肌纤维化和心功能。方法:将40只大鼠随机分成4组各10只:对照组(C组)大鼠予单次腹腔注射生理盐水,抗纤益心方组(AF组)大鼠在C组基础上加用抗纤益心方溶液灌胃,DC组大鼠予单次腹腔注射链脲佐菌素制作DC模型,DC+AF组大鼠予单次腹腔注射链脲佐菌素和抗纤益心方溶液灌胃处理;观察4组大鼠经过不同处理后心功能及心肌纤维化变化情况。结果:DC组与DC+AF组大鼠血糖浓度明显高于C组(P<0.05);DC组大鼠左室舒张末压(LVEDP)显著上升(P<0.05),左室收缩压(LVSP)显著下降(P<0.05),DC+AF组大鼠LVSP明显高于DC组(P<0.05),LVEDP低于DC组(P<0.05);DC组与DC+AF组大鼠Ⅰ型胶原水平明显高于C组(P<0.05),DC+AF组大鼠Ⅰ型胶原水平显著低于DC组(P<0.05);DC组大鼠TGF-β1、Smad2及Smad3的磷酸化水平显著高于C组(P<0.05),而DC+AF组大鼠TGF-β1、Smad2及Smad3的磷酸化水平明显低于DC组(P<0.05)。结论:抗纤益心方对DC大鼠心肌纤维化具有一定的抑制作用,并能改善大鼠心功能,其作用机制与TGF-β/Smads信号系统密切相关。Objective： To investigate the influence of Kangxian Yixin prescription on anti-cardiac fibrosis and improvement of cardiac function in diabetic cardiomyopathy rats through transforming growth factor β/smads signal pathway. Methods： Forty rats were randomly divided into 4 groups,i. e.,control group（C group）,anti-fibrinolytic group（AF group）,diabetic cardiomyopathy group（DC group） and DC ＋ AF group with 10 rats in each group. Rat models were induced by intraperitoneal injection of streptozotocin. After the models were established,the rats were treated with Kangxian Yixin prescription for 8 weeks by gavage. Rats in C group were given a single intraperitoneal injection of saline. In addition to a single intraperitoneal injection of saline,the rats in AF group were given Kangxian Yixin prescription by gavage. The rats were treated with streptozotocin by single intraperitoneal injection in DC group. Besides a single intraperitoneal injection of streptozotocin,the rats were additionally given Kangxian Yixin prescription by gavage in DC ＋ AF group. The changes of cardiac function and cardiac fibrosis after different treatment were observed in the four groups. Results： The blood glucose concentration in DC group and DC ＋ AF group was significantly higher than that in C group（P 〈 0. 05）. The level of left ventricular end diastolic pressure（LVEDP） was significantly increased（P 〈 0. 05）,and the level of left ventricular systolic pressure（LVSP） was significantly decreased（P 〈 0. 05）. The LVSP in DC ＋ AF group was higher than that in DC group（P 〈 0. 05）. The LVEDP in DC ＋ AF group was lower than that in DC group（P 〈 0. 05）. The collagen-Ⅰ expression in DC group and DC ＋ AF group was higher than that in C group（P 〈 0. 05）,while the expression in DC group was higher than that in DC ＋ AF group（P 〈 0. 05）. The phosphorylation of TGF-β1,Smad2 and Smad3 in DC group was higher than that in C group（P 〈 0. 05） and DC ＋ AF group（P 〈 0. 05�

关 键 词：转化生长因子-β/Smads信号通路 抗纤益心方 糖尿病心肌病 心肌纤维化 心功能 

分 类 号：R-33[医药卫生] R587.2