粉防己碱对胃癌耐药细胞ZNF139、MDR1及GST-π mRNA的影响  

Effects of TET on the expression of ZNF139,MDR1 and GST-π mRNA in multidrug resistant gastric cancer cells

作　　者：张旭[1] ZHANG Xu(Digestive Department of the First People’s Hospital of Neijiang, Neijiang 641000, Chin)

机构地区：[1]内江市第一人民医院消化内科,四川内江641000

出　　处：《东南大学学报（医学版）》2018年第3期467-470,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:探讨粉防己碱(TET)对胃癌耐药细胞锌指蛋白139(ZNF139)、多耐药基因1(MDR1)及谷胱甘肽S转移酶-π(GST-π)mRNA的影响。方法:选取胃癌细胞SGC7901以及胃癌耐阿霉素细胞SGC7901/ADR,采用逆转录-聚合酶链反应和蛋白质印迹法检测两种细胞ZNF139、MDR1、GST-πmRNA及蛋白表达,同时检测2.0!g·ml-1TET处理前后SGC7901/ADR细胞ZNF139、MDR1、GST-πmRNA及蛋白表达。结果:SGC7901/ADR细胞ZNF139、MDR1、GST-πmRNA相对表达量明显高于SGC7901细胞(P<0.05);SGC7901/ADR细胞ZNF139、MDR1、GST-π蛋白相对表达量明显高于SGC7901细胞(P<0.05);TET处理后SGC7901/ADR细胞ZNF139、MDR1、GST-πmRNA及蛋白相对表达量均较处理前明显降低(P<0.05)。结论:TET能下调胃癌耐药细胞ZNF139、MDR1、GST-πmRNA和蛋白表达水平,从而在逆转胃癌多药耐药特性方面有重要作用。Objective： To investigate the effects of tetrandrine（TET） on zinc finger protein 139（ZNF139）,multidrug resistance gene 1（MDR1） and glutathione S transferase-π（GST-π） mRNA of multidrug resistant gastric cancer cells. Methods： Gastric cancer cells SGC7901 and SGC7901/ADR were selected,and ZNF139,MDR1 and GST-π mRNA and protein expression in the two kinds of cells were detected by RT-PCR and Western blot.ZNF139,MDR1 and GST-π mRNA and protein expression of SGC7901/ADR cells were detected before and after2. 0 !g·ml-1 TET treatment. Results： ZNF139,MDR1 and GST-π mRNA relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells（P 〈 0. 05）; ZNF139,MDR1 and GST-π protein relative expression of SGC7901/ADR cells were significantly higher than those of SGC7901 cells（P 〈 0. 05）; After TET treatment ZNF139,MDR1 and GST-π mRNA and protein relative expression of SGC7901/ADR cells were significantly decreased compared with those before treatment（P 〈 0. 05）. Conclusion： TET can down-regulate ZNF139,MDR1 and GST-π mRNA and protein expression in drug resistant gastric cancer cells,which plays an important role in reversing multidrug resistance characteristics of gastric cancer.

关 键 词：胃癌 粉防己碱 锌指蛋白139 多耐药基因1 谷胱甘肽S转移酶-Π 耐药 

分 类 号：R735.2[医药卫生—肿瘤]