实验用狨猴微卫星引物筛选及遗传多样性分析  

Microsatellite marker design and genetic analysis for the common marmoset

作　　者：滕永康[1] 刘先菊[1] 张旭 向志光[1] 阮研硕[1] 肖冲[1] 刘云波[1] TENG Yongkang;LIU Xianju;ZHANG Xu;XIANG Zhiguang;RUAN Yanshuo;XIAO Chong;LIU Yunbo(Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences,Beijing 100021,China)

机构地区：[1]中国医学科学院医学实验动物研究所,北京100021

出　　处：《中国比较医学杂志》2018年第6期15-20,28,共7页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划(2017YFA0105201);北京市科技计划(Z161100000216154)

摘　　要：目的筛选、优化狨猴微卫星DNA引物,用以对中国医学科学院医学实验动物研究所引进的实验用狨猴种群进行遗传学分析及评估。方法用筛选的20对狨猴微卫星引物,对随机抽取的30只狨猴血液样本进行基因组DNA提取及PCR扩增,扩增产物经电泳鉴定后进行STR扫描检测,用Popgene1.32软件对STR扫描结果进行数据处理和分析。结果 20对微卫星引物均呈现出遗传多样性,共检测147个等位基因,观察等位基因数5~10个,平均7.35个;有效等位基因数2.2500~6.3830个,平均4.0402个;观察杂合度0.000~0.4667,平均0.1533;期望杂合度0.1424~0.4350,平均0.2506;香隆指数1.2242~2.0324,平均1.5949;多态信息含量0.5366~0.8254,平均0.7053。结论本文筛选的20对狨猴微卫星引物具有高度遗传多样性,均处于Hardy-Weinberg平衡状态。Objective To screen and optimize the microsatellite DNA primers of the laboratory commonmarmoset, analyze and evaluate the population genetic quality for the marmosets （Callithrix jacchus） introduced into theInstitute of Medical Laboratory Animal science, Chinese Academy of Medical Sciences. Methods A total of 30 marmosetswere randomly chosen, and their genome DNA from blood was extracted using phenol / chloroform method. Themicrosatellite DNA was amplified using standard polymerase chain reaction （PCR）. The amplification products were testedby STR scanning after 2% agrose gel and 8% PAGE electrophoresis. The data processing and genetic analysis werecompleted using the Popgene1. 32 software. Results A total of 20 pairs of microsatellite loci showed geneticpolymorphism, and 147 alleles were detected. The number of allele was 5 to 10, average 7. 35. The effective allele was2. 2500 to 6. 3830, average 4. 0402. The observed heterozygosity was 0. 000 to 0. 4667, average 0. 1533. The expectedheterozygosity was 0. 1424 to 0. 4350, average 0. 2506. The Shannon diversity index was 1. 2242 to 2. 0324, average1. 5949. The polymorphic information content was 0. 5366 to 0. 8254, average 0. 7053. Conclusions The 20 pairs ofmarmoset microsatellite primers are genetically highly diverse and are in a Hardy.Weinberg equilibrium.

关 键 词：实验用狨猴 微卫星DNA 遗传多样性 

分 类 号：R-33[医药卫生]