注射用骨肽对去卵巢骨质疏松大鼠骨微结构、骨密度和骨生物力学性能的影响  

Effects of ossotide injection on bone mineral density(BMD),bone microstructure and biomechanical properties in ovariectomized rats

作　　者：韩龙[1] 吴水培[2] HAN Long;WU Shuipei(No.89 Clinical College of PLA,Medical University of Anhui,Huzhou 313000,China;No.89 Hospital of PLA,Huzhou 313000)

机构地区：[1]安徽医科大学解放军九八临床学院,浙江湖州313000 [2]解放军九八医院,浙江湖州313000

出　　处：《中国比较医学杂志》2018年第6期79-83,共5页Chinese Journal of Comparative Medicine

摘　　要：目的观察注射用骨肽对去卵巢骨质疏松大鼠骨密度、骨生物力学和小肠钙结合蛋白(CaBp-D9K)mRNA表达的影响,探讨注射用骨肽治疗去卵巢骨质疏松的作用机制。方法将建模成功(切除双除卵巢)的48只大鼠随机分为观察组和模型组,另分别取24只大鼠作为假手术组(切除部分小肠系膜)和空白组。空白组、假手术组和模型组给予生理盐水灌胃,观察组给予注射用骨肽1.1 mg/kg灌胃。干预2个月后观察四组的骨体积(BV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)、骨小梁分离度(Tb.Sp)、骨密度、骨生物力学、血清1,25(OH)_2D_3和小肠CaBp-D9K mRNA表达水平。结果模型组骨密度、最大载荷、断裂载荷、BV、Tb.Th、Tb.N、血清1,25(OH)_2D_3和小肠CaBp-D9K mRNA表达水平低于空白组和假手术组,Tb.Sp高于空白组和假手术组,组间比较差异有显著性(P<0.05)。观察组骨密度、最大载荷、断裂载荷、BV、Tb.Th、Tb.N、血清1,25(OH)_2D_3和小肠CaBpD9K mRNA表达水平高于模型组,Tb.Sp低于模型组,组间比较差异有显著性(P<0.05)。结论注射用骨肽可降低去卵巢大鼠骨质疏松程度,增加小肠CaBp-D9k mRNA表达,促进肠钙吸收可能是其重要作用机制。Objective To observe the effect of ossotide injection on bone mineral density （ BMD）, bonemicrostructure and biomechanical properties and mRNA expression of small intestinal calcium binding protein （ CaBp-D9K）, and to study the mechanism of ossotide injection in the treatment of ovariectomized osteoporosis. Methods Forty-eight 3-month old SPF male rats with successful modeling （ excision of bilateral ovaries） were randomly divided into theobservation group and model group, 24 normal rats were divided into sham operation group （excised part of the mesentericmembrane）, and 24 normal blank group. The blank group, sham operation group and model group were given normalsaline, and the observation group was intragastrically given 1- 1 mg / kg ossotide. Two months after intervention, the bonevolume （BV）, trabecular bone volume （ Tb. Th）, trabecular number （ Tb. N）, trabecular separation （ Tb. Sp）, bone mineral density, bone biomechanics, serum 1, 25 （ OH）2D3 levels and CaBp-D9K mRNA expression levels of smallintestine were assessed and statistically analyzed. Results The bone mineral density, maximum load, fracture load, BV,Tb. Th, Tb. N, serum 1, 25 （ OH） 2D3 and intestinal CaBp-D9K mRNA expression level in the model group weresignificantly lower than those of the control group and sham operation group （P 〈 0.05）, Tb. Sp of the model group wassignificantly higher than that of the control group and sham operation group （ P 〈 0.05）. The bone mineral density,maximum load, fracture load, BV, Tb. Th, Tb. N, serum 1,25（OH）2D3 and intestinal CaBp-D9K mRNA expression levelin the observation group were significantly higher than those of the model operation group, and the Tb. Sp of the observationgroup was lower than that of the model group （P 〈 0.05）. Conclusions Ossotide injection treatment can reduce thedegree of osteoporosis in ovariectomized rats, increasing intestinal CaBp-D9k mRNA expression and promoting intestinalcalcium absorption may be its important mecha

关 键 词：骨质疏松 绝经 注射用骨肽 骨密度 骨生物力学 钙结合蛋白 大鼠 

分 类 号：R-33[医药卫生]