采用木屑培养基传代的金针菇菌种稳定性  

Stability of Flammulina velutipes Spawn Subcultured on a Sawdust Medium

作　　者：张丹[1] 朱爱莲 赵静 周敏 张引芳 尚晓冬[1] 王瑞娟[1] 谭琦[1] ZHANG Dan;ZHU Ailian;ZHAO Jing;ZHOU Min;ZHANG Yinfanga;SHANG Xiaodong;WANG Ruijuan;TAN Qi(Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization（South）,Ministry of Agriculture,P.R.China National Engineering Research Center of Edible Fungi,National R＆D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;Shanghai Bright Esunyes Bio-tech Co.,Ltd,Shanghai 201400,China)

机构地区：[1]上海市农业科学院食用菌研究所农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心国家食用菌加工技术研发分中心上海市农业遗传育种重点开放实验室,上海201403 [2]上海光明森源生物科技有限公司,上海201400

出　　处：《食用菌学报》2018年第2期29-34,共6页Acta Edulis Fungi

基　　金：上海市食用菌产业技术体系建设专项[沪农科产字(2017)第9号];上海市市级农口系统青年人才成长计划项目[沪农青字(2017)第1-11号]

摘　　要：对金针菇(Flammulina velutipes)菌种在木屑培养基上进行连续6次的继代培养,比较不同继代菌种与原始母种的菌丝生长速度、液体发酵pH以及工厂化栽培子实体的相关农艺性状的变化,利用基因组重测序技术检测继代菌种在DNA分子水平的遗传变异。结果显示:与原始母种相比,各继代菌种的表型性状无显著性差异,6次传代菌种的表型性状均稳定。DNA分子水平的遗传分析表明,第1~5次继代菌种基因组中的遗传变异呈缓慢增长趋势。与原始母种相比,第5次继代菌种的遗传差异达到7.92%,其它继代菌种的遗传差异均低于4%。采用木屑培养基进行金针菇菌种的继代培养可有效保持菌种的稳定性。The stability of Flammulina velutipes spawn subcultured on a sawdust medium was studied. The original strain was subcultured for six consecutive passages and then each subculture was compared to the original starting strain in terms of mycelial growth rate, pH in liquid fermentation, agronomic traits of fruiting body pertaining to the factory production of F. velutipes, and genetic deviations at the DNA level. Compared to the original strain, all the six passages of strains maintained high stability in phenotypic traits, and genetic variations for the first five passages gradually increased as the number of subcultures increased. Compared to the original strain, genetic differences of the first four subcultures remained less than 4%, and the genetic difference of the fifth subculture was 7.92%. This experiment showed that the spawn quality of F. velutipes was effectively maintained on the sawdust medium.

关 键 词：金针菇 继代培养 农艺性状 种性控制 

分 类 号：S646.15[农业科学—蔬菜学]