前列腺素E1干预大鼠骨髓间充质干细胞的旁分泌和迁移  

Effect of prostaglandin E1 on paracrine and migration of rat bone marrow mesenchymal stem cells

作　　者：曾宽[1] 张露 邓保平[1] 高敏楠 江慧琦[1] 王萌 邓宇斌[2] 杨艳旗[1] Zeng Kuan;Zhang Lu;Deng Bao-ping;Gao Min-nan;Jiang Hui-qi;Wang Meng;Deng Yu-bin;Yang Yan-qi(Department of Cardiothoracic Surgery,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,Guangzhou 510120,Guangdong Province,China;Translational Medicine Center,the First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,Guangdong Province,China)

机构地区：[1]中山大学孙逸仙纪念医院心脏外科,广东省广州市510120 [2]中山大学附属第一医院转化医学中心,广东省广州市510080

出　　处：《中国组织工程研究》2018年第17期2656-2660,共5页Chinese Journal of Tissue Engineering Research

基　　金：中山大学百人计划专项基金(F002009011);广东省企业技术研发与升级改造专项基金(第一批)(2013B021800102)~~

摘　　要：背景:目前还缺乏前列腺素E1对骨髓间充质干细胞旁分泌和迁移影响的研究。目的:观察前列腺素E1与骨髓间充质干细胞共培养对细胞旁分泌和迁移的影响。方法:取第3代骨髓间充质干细胞与10μg/L前列腺素E1共培养,在3,6,9,12,24,48,72 h各时间点收集培养孔内上清液100μL,利用酶联免疫吸附法检测不同时间骨髓间充质干细胞分泌血管内皮生长因子水平。采用细胞划痕实验及Transwell迁移实验观察前列腺素E1干预后骨髓间充质干细胞的迁移能力。结果与结论:(1)前列腺素E1作用3 h后骨髓间充质干细胞分泌血管内皮生长因子水平开始升高,作用24 h时血管内皮生长因子分泌达到峰值,随后逐渐下降;(2)细胞划痕实验和Transwell迁移实验显示,前列腺素E1作用后迁移的骨髓间充质干细胞显著增多(P<0.05);(3)结果可见,前列腺素E1可促进骨髓间充质干细胞分泌血管内皮生长因子,并能提高它的迁移能力。BACKGROUND： Currently, there are few studies about prostaglandin E1 in the paracrine and migration of bone marrow mesenchymal stem cells. OBJECTIVE： To explore the effects of prostaglandin E1 in the paracrine and migration of bone marrow mesenchymal stem cells. METHODS： Bone marrow mesenchymal stem cells isolated from Sprague Dawley rats were cultured in vitro. Passage 3 cells were co-cultured with prostaglandin E1 at concentrations of 10 μg/L, and then culture supernatant was collected at 3, 6, 9, 12, 24, 48, and 72 hours after co-culture. The level of vascular endothelial growth factor was detected by enzymelinked immunosorbent assay. Effects of prostaglandin E1 on the migration of bone marrow mesenchymal stem cells were detected by Transwell assay and cell scratch assay. RESULTS AND CONCLUSION： After treatment with prostaglandin E1 for 3 hours, bone marrow mesenchymal stem cells began to secrete vascular endothelial growth factors, and the secretion level was peaked at 24 hours and then gradually decreased. Results from the Transwell assay and cell scratch assay showed that the migration ability of bone marrow mesenchymal stem cells was significantly promoted by prostaglandin E1（P〈0.05）. Overall findings reveal that prostaglandin E1 promotes the secretion of vascular endothelial growth factor from bone marrow mesenchymal stem cells and enhances cell migration.

关 键 词：骨髓 间质干细胞 前列腺素E类 血管内皮生长因子类 细胞运动 组织工程 骨髓间充质干细胞 前列腺素E1 旁分泌 迁移 血管内皮生长因子 干细胞 

分 类 号：R394.2[医药卫生—医学遗传学]