力学因素对间充质干细胞神经向分化的影响  

Effects of mechanical stresses on neural differentiation of mesenchymal stem cells

作　　者：江静怡 樊瑜波[1,2] 郑丽沙[1,2] Jiang Jing-yi;Fan Yu-bo;Zheng Li-sha(Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,School of Biological Science and Medical Engineering,Beihang University,Beijing 100083,China;Beijing Advanced Innovation Centre for Biomedical Engineering,Beihang University,Beijing 100083,China)

机构地区：[1]北京航空航天大学生物与医学工程学院,生物力学与力生物学教育部重点实验室,北京市100083 [2]北京航空航天大学生物医学工程高精尖创新中心,北京市100083

出　　处：《中国组织工程研究》2018年第17期2761-2768,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(11572030,61227902,11120101001,11421202); 科技部国家重点研发计划(2017YFC0108505,2017YFC0108500)

摘　　要：背景:目前,通过对细胞施加机械力学刺激或改变细胞所处的微环境来调控干细胞的分化方向,已经成为了组织工程与再生医学领域的研究热点。目的:综述了近10年来力学因素调控间充质干细胞神经向分化领域的研究进展,为干细胞替代疗法治疗退行性疾病提供相关的理论基础。方法:检索Web of Science和Pub Med数据库中关于力学因素对间充质干细胞神经向分化影响的文献,并进行系统的归纳总结和分析,最终得到104篇相关文献。结果与结论:机械力刺激、细胞外物理环境因素对骨髓间充质干细胞、脂肪间充质干细胞、牙髓干细胞、子宫内膜干细胞等间充质干细胞的神经向分化有重要影响。不同的力学因素可能通过PI3K-AKT-m TOR、Wnt/β-catenin、Rho、MAPK等信号通路调控间充质干细胞的神经向分化,但具体的机制还有待进一步研究。BACKGROUND： Currently, regulation of stem cell differentiation by mechanical stresses or microenvironment has become a popular issue in the tissue engineering and regenerative medicine. OBJECTIVE： To review the progress in the effects of mechanical stress on neural differentiation of mesenchymal stem cells in the last decade, providing theoretical basis for stem cell replacement therapy in the treatment of degenerative diseases. METHODS： A search of Web of Science and Pub Med database was done to retrieve articles addressing the effects of mechanical stresses on the neural differentiation of mesenchymal stem cells. After systematical analysis and summarization, 104 articles were finally selected and analyzed. RESULTS AND CONCLUSION： Mechanical stimulation and extracellular physical environment factors have important influence on the neural differentiation of mesenchymal stem cells from the bone marrow, adipose tissues, dental pulp and endometrium. PI3 K-AKT-m TOR, Wnt/beta-catenin, Rho, MAPK signaling pathways may be involved in the mechanical stresses regulated neural differentiation. However, the clear molecular mechanism needs further studies.

关 键 词：间质干细胞 细胞分化 神经元 应力 物理 组织工程 机械应力 细胞外基质 三维培养 间充质干细胞 神经向分化 干细胞 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]