16S rDNA克隆文库法分析油藏细菌群落结构多样性  

Analysis of Bacterial Community Diversity in the Oil Reservoir by 16S rDNA Clone Library Method

作　　者：万真真 WAN Zhenzhen(School of Resources and Environment,Anqing Normal University,Anqing 246133,China)

机构地区：[1]安庆师范大学资源环境学院,安徽安庆246133

出　　处：《安庆师范大学学报（自然科学版）》2018年第2期63-67,共5页Journal of Anqing Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(41603083);安徽省教育厅重点项目(KJ2016A432)

摘　　要：本文利用细菌16S rDNA克隆文库法对大港油田油藏内源微生物群落结构进行系统分析。文库构建结果表明,油藏细菌由8个门类构成,分别为Alphaproteobacteria(46%)、Betaproteobacteria(35%)、Firmicutes(8%)、Nitrospirae(2%)、Synergistetes(1%)、Dictyoglomia(1%)、Deferribacteres(1%)、Gammaproteobacteria(1%)以及Unclassified bacteria。其中,存在如鞘胺醇单胞菌(Sphingomonas)、代尔夫特菌(Delftia)等大量具有石油烃降解功能的细菌种属。探索极端油藏环境中微生物群落结构特征,为微生物驱油技术的开发奠定了良好的基础。As one significant microbial resource, microorganisms play an important role in crude oil exploitation and remediation of oil-contaminated soil. 16 S rDNA clone library was conducted to explore the bacterial community of oil reservoir in this research. Based on clone library analysis, bacteria mainly belong to 8 phyla in oil reservoir, and they are Alphaproteobacteria（46%）, Betaproteobacteria（35%）, Firmicutes（8%）, Nitrospirae（2%）, Synergistetes（1%）, Dictyoglomia（1%）,Deferribacteres（1%）, Gammaproteobacteria（1%）, and unclassified bacteria. Meanwhile, there are many hydrocarbon-degrading bacteria, such as genus Sphingomonas and genus Delftia in oil reservoir. Better understanding bacterial community in extreme oil reservoirs will construct a good foundation for microbial enhanced oil recovery（MEOR）.

关 键 词：油藏 细菌 克隆文库 微生物驱油 

分 类 号：X172[环境科学与工程—环境科学]