常见野生蘑菇的红外光谱及其二维相关红外光谱的鉴别  

Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy

作　　者：马殿旭 刘刚[1] 欧全宏[1] 于海超[1] 李会梅[1] 时有明[2] MA Dian-xu;LIU Gang;OU Quan-hong;YU Hai-chao;LI Hui-mei;SHI You-ming(School of Physics and Electronic Information,Yunnan Normal University,Yunnan Key Lab of Optoelectronic Information,Kunming 650500,China;School of Physics and Electronic Engineering,Qujing Normal University,Qujing 655011,China)

机构地区：[1]云南师范大学物理与电子信息学院,云南省光电信息技术重点实验室,云南昆明650500 [2]曲靖师范学院物理与电子工程学院,云南曲靖655011

出　　处：《光谱学与光谱分析》2018年第7期2113-2122,共10页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31760341); 云南省高校科技创新团队支持计划资助

摘　　要：采用傅里叶变换红外光谱结合相关性分析、二阶导数谱和二维相关谱对13种常见野生蘑菇进行鉴别研究。结果显示13种野生蘑菇的红外光谱光谱特征相似,主要由蛋白质、多糖的吸收组成,13种样品的相关系数最小为0.779,最大值为0.960。在1 700~1 400和1 400~800cm-1范围的二阶导数光谱中,各样品吸收峰的强度、位置和形状均有明显差异。二维相关红外光谱在1 380~1 680cm-1范围的强自动峰整体相近,但自动峰和交叉峰位置、形状存在差异;在920~1 230cm-1的范围,各样品的自动峰和交叉峰的数量、强度和位置差异明显。结果表明傅里叶变换红外光谱结合相关性分析、二阶导数谱和二维相关谱有望发展为区分不同种类蘑菇的快速方法。Fourier Transform Infrared Spectroscopy combined with correlation analysis,second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy was used to discriminate 13 species of wild mushrooms.The results showed that the general characteristics of absorption bands in the original spectra were similar,which were mainly composed of protein and polysaccharides.Correlation analysis was applied for the spectra of samples,and the correlation coefficient between Termitomyces eurrhizus and Tylopilus plumbeoviolaceoides was 0.779,which was the minimum,while the correlation coefficient between Pleurotus lignatilis Gill and Hygrophorus lucorum Kalchbr was 0.960,which was the maximum.Coprinus comatus had little correlation with other samples.The differences of intensity,position and shape were observed in second derivative spectra in the range of 1 700～1 400 and 1 400～800 cm^-1.In two-dimensional correlation spectra,the differences of the position or form of auto-peaks and cross peaks have been found in the range of 1 380～1 680 cm^-1.The number,position and intensity of auto-peaks and cross peaks were obviously different in the range of 920～1 230 cm^-1.The study demonstrates that Fourier Transform Infrared spectroscopy combined with correlation analysis,second derivative infrared spectroscopy and two-dimensional correlation infrared spectroscopy might be developed as a rapid method to discriminate different kinds of mushrooms.

关 键 词：野生蘑菇 傅里叶变换红外光谱(FTIR) 相关性分析 二维相关红外光谱 鉴别 

分 类 号：O657.3[理学—分析化学]