人源肺癌循环肿瘤细胞系在不同品系小鼠上肺转移能力研究  

Study on lung metastasis of human lung cancer circulating tumor cell lines in different strains of mice

作　　者：阙祖俊[1,2] 董昌盛[1,2] 罗斌[1,2] 田建辉[1,2] QUE Zu-Jun;DONG Chang-Sheng;LUO Bin;TIAN Jian-Hui(Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Oncology Institute of Traditional Chinese Medicine,Shanghai Institute of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属龙华医院 [2]上海市中医药研究院中医肿瘤研究所,上海200032

出　　处：《转化医学电子杂志》2018年第5期26-28,共3页E-Journal of Translational Medicine

基　　金：国家自然科学基金面上项目(81373621);上海市市级医疗卫生优秀学科带头人培养计划(2017BR044);上海市青年科技英才扬帆计划(17YF1419700);龙医学者-育苗计划(LYTD-39)

摘　　要：目的:探讨一种尾静脉注射人源肺癌循环肿瘤细胞建立的转移模型,比较NOD/SCID小鼠、Nude小鼠和C57BL/6小鼠对该模型的敏感性。方法:将人源肺癌循环肿瘤细胞通过尾静脉注射接种到C57BL/6小鼠、Nude小鼠和NOD/SCID小鼠体内,在不同时间节点对不同品系小鼠上的肺转移情况进行评估;H&E染色检测肺组织病理变化。结果:CTC-TJH-01细胞接种于NOD/SCID小鼠体内后,在第8周首次观察到肺部成瘤,在第10周小鼠肺部成瘤率为100%。而在Nude小鼠和C57BL/6小鼠体内均不成瘤。结论:人源肺癌循环肿瘤细胞通过尾静脉注射接种于NOD/SCID小鼠后能够发生肺转移,该模型可用于临床抗转移药物筛选与评价研究。Objective： To investigate a metastasis model established by tail vein injection of human lung cancer circulating tumor cells,and compare the sensitivity of NOD/SCID mice,nude mice and C57 BL/6 mice to this model. Methods： Human lung cancer circulating tumor cells were inoculated into C57 BL/6 mice,Nude mice,and NOD/SCID mice via tail vein injection. The lung metastases of different strains of mice were assessed at different time points; HE staining was used to detect the pathological changes of lung tissue. Results：After CTC-TJH-01 cells were inoculated into NOD/SCID mice,the lung tumors were observed for the first time at the 8 th week. In the 10 th week,the rate of tumor formation in the lungs of mice was 100%. However,tumors were not formed in Nude mice and C57 BL/6 mice. Conclusion： Human lung cancer circulating tumor cells can invade lung metastases after inoculation in NOD/SCID mice by tail vein injection.This model can be used for the screening and evaluation of clinical anti-metastatic drugs.

关 键 词：循环肿瘤细胞 肺癌 转移 动物模型 

分 类 号：R734.2[医药卫生—肿瘤]