MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响  

Effect of micro RNA-150 on proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells

作　　者：李文辉[1] 吕博文 钱钧[2] 苏丽菊[3] 杨桐树[1] 钱景荣 王杰 LI Wenhui;LV Bowen;QIAN Jun;SU Liju;YANG Tongshu;QIAN Jingrong;WANG Jie(Harbin Medical University Cancer Hospital,Harbin 150081,China;Harbin Medical University;Harbin First Hospita)

机构地区：[1]哈尔滨医科大学附属肿瘤医院,哈尔滨150081 [2]哈尔滨医科大学 [3]哈尔滨市第一医院

出　　处：《实用肿瘤学杂志》2018年第3期208-213,共6页Practical Oncology Journal

基　　金：黑龙江省留学归国人员科学基金(编号:LC2015035)

摘　　要：目的研究micro RNA-150(mi R-150)在人上皮性卵巢癌细胞中的表达情况,及其对人上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响。方法通过荧光实时定量PCR(q RT-PCR)检测各处理组细胞中的mi R-150表达水平;利用MTT法、流式细胞技术、Transwell法探究mi R-150的表达对上皮性卵巢癌细胞增殖、凋亡及侵袭转移能力的影响。结果与正常卵巢上皮细胞(T29)相比,上皮性卵巢癌细胞(A2780和OVCAR3)中mi R-150表达显著降低(P<0.01);转染mi R-150mimic后,A2780和OVCAR3细胞中mi R-150水平明显升高(P<0.01);MTT试验显示,转染培养三天后,mi R-150 mimic组细胞OD值(A2780:1.12±0.03;OVCAR3:1.91±0.03)较Blank组(A2780:2.35±0.09;OVCAR3:2.63±0.07)及mi R-150 NC组(A2780:2.18±0.07;OVCAR3:2.43±0.11)明显降低(P<0.01);细胞凋亡检测显示:mi R-150 mimic组凋亡率(A2780:16.10±0.58%;OVCAR3:15.16±1.30%)较Blank组(A2780:10.07±0.66%;OVCAR3:3.81±0.24%)及mi R-150 NC组(A2780:10.36±1.08%;OVCAR3:4.89±0.07%)明显升高(P<0.01);Transwell试验显示:mi R-150 mimic组穿膜细胞数(A2780:38.67±2.03;OVCAR3:28.67±2.03)较Blank组(A2780:76.30±7.45;OVCAR3:55.67±3.18)及mi R-150 NC组(A2780:74.33±5.78;OVCAR3:56.33±3.84)明显减少(P<0.01)。结论 mi R-150在上皮性卵巢癌细胞中表达降低,可能是上皮性卵巢癌增殖、侵袭转移的机制之一;上调mi R-150的表达可以抑制上皮性卵巢癌细胞增殖、促进细胞凋亡,并且降低上皮性卵巢癌细胞侵袭转移能力。Objective The aim of this study was to investigate the expression of micro RNA-150（ mi R-150） in human epithelial ovarian cancer cells and its effect on proliferation,apoptosis,invasion and metastasis of human epithelial ovarian cancer cells.Methods The expression level of mi R-150 in cells from each treatment group was detected by Real-Time PCR（ q RT-PCR）;effects of proliferation,apoptosis,invasion and metastasis of epithelial ovarian cancer cells was investigated by MTT,flow cytometry,and transwell assays. Results Compared with normal ovarian epithelial cells（ T29）,the expression of mi R-150 was significantly decreased in epithelial ovarian cancer cells（ A2780 and OVCAR3）（ P〈 0. 01）; After transfection mi R-150 mimic,the expression of mi R-150 in A2780 and OVCAR3 cells was significantly increased（ P〈 0. 01）; After 3 d of transfection,the OD values of the mi R-150 mimic group（ A2780： 1. 12 ± 0. 03; OVCAR3： 1. 91 ± 0. 03） were lower than that in the blank group（ A2780： 2. 35 ± 0. 09;OVCAR3： 2. 63 ± 0. 07） and the mi R-150 NC group（ A2780： 2. 18 ± 0. 07; OVCAR3： 2. 43 ± 0. 11）（ P〈 0. 01）; The apoptotic rate in the mi R-150 mimic group（ A2780： 16. 10 ± 0. 58%; OVCAR3： 15. 16 ± 1. 30%） were significantly increased when compared to the blank group（ A2780： 10. 07 ± 0. 66%; OVCAR3： 3. 81 ± 0. 24%） and the mi R-150 NC group（ A2780： 10. 36 ± 1. 08%;OVCAR3： 4. 89 ± 0. 07%）（ P〈 0. 01）; The number of transmembrane cells in the mi R-150 mimic group（ A2780： 38. 67 ± 2. 03;OVCAR3： 28. 67 ± 2. 03） was higher than that in the blank group（ A2780： 76. 30 ± 7. 45; OVCAR3： 55. 67 ± 3. 18） and the mi R-150 NC group（ A2780： 74. 33 ± 5. 78; OVCAR3： 56. 33 ± 3. 84）（ P〈 0. 01）. Conclusion The decreased expression of mi R-150 in epithelial cancer cells may be one of the mechanisms of proliferation,invasion and metastasis of epithelial ovarian cancer. Up-regulation of mi R-150 may inhibit the proliferation

关 键 词：上皮性卵巢癌细胞 miR-150 增殖 凋亡 侵袭转移 

分 类 号：R737.31[医药卫生—肿瘤]