CdTe量子点与微囊藻毒素-LR抗体的偶联及其相互作用的研究  

Study of the Interaction Between Water-Soluble CdTe Quantum Dots(QDs)and Microcystin-LR Antibody-QDs

作　　者：李莹[1] 孙嘉笛 孙秀兰[1,2] 张银志[2] LI Ying;SUN Jiadi;SUN Xiulan;ZHANG Yinzhi(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]食品科学与技术国家重点实验室,江南大学,江苏无锡214122

出　　处：《食品与生物技术学报》2018年第5期463-471,共9页Journal of Food Science and Biotechnology

基　　金：国家自然基金-广东省联合基金项目(U13012141); 国家973计划项目(2012CB720804); 公益性行业(农业)科研专项(201120130)

摘　　要：以巯基乙酸作为稳定剂制备了水溶性CdTe量子点,将纯化后的量子点与微囊藻毒素-LR抗体偶联,通过分析紫外-可见吸收光谱和荧光光谱,研究了不同温度下CdTe量子点与微囊藻毒素-LR抗体的相互作用,得到了量子点与抗体之间的表观结合常数Kapp(84.3M-1),SternVolmer猝灭常数KSV(6.74×104(298 K),6.11×104(304 K),5.93×104(310 K)),结合位点n和结合常数K。此外,还研究了量子点与抗体之间相互作用的热力学参数△H(<0),△G(<0)和△S(>0),经实验数据发现,量子点与抗体之间的作用力以静电相互作用为主,氢键、范德华力也起到了一定的作用。Water-soluble CdTe quantum dots（QDs） were synthesized using thioglycollic acid as a stabilizer,and the purified QDs were bind to the Microcystin-LR antibody. The interaction between the QDs and the Microcystin-LR antibody at different temperatures were investigated. The association constant Kapp（84.3M^-1）,the Stern-Volmer quenching constant Ksv（6.74×10^4（298 K）,6.11×10^4（304 K）,5.93×10^4（310 K））,the number of binding sites n,and the binding constant K were all calculated through the analysis of UV-Vis absorption and fluorescence spectrum. In addition,the interaction of the thermodynamic parameters between the QDs and the antibody were studied ,△H（〈0）,△G（〈0）and △S（〉0）,indicating that electrostatic played mainly roles in the interactions between CdTe QDs and Microcystin-LR antibody,and the hydrogen bond and van der Waals also made the contributions.

关 键 词：量子点 微囊藻毒素-LR抗体 光谱 热力学参数 

分 类 号：R392.11[医药卫生—免疫学]