灵芝孢子粉多糖的分离纯化、结构表征及免疫活性初探  

Purification,Structural Characterigation and Immunological Activity of Polysaccharides from the Spores of Ganoderma lucidum

作　　者：冯蒙蒙 王海鸣 杨惠成 孙震[3] 孙秀兰[3] 戴军 FENG Mengmeng;WANG Haimin;YANG Huicheng;SUN Zhen;SUN Xiulan;DAI Jun(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Guangzhou GRG Metrology ＆ Test Co.,LTD,Guangzhou 510065,China;Food Academy of Jiangnan University,Wuxi 214122,China)

机构地区：[1]食品与技术国家重点实验室,江南大学,江苏无锡214122 [2]广州广电计量检测股份有限公司,广东广州510065 [3]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2018年第5期502-508,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31171688)

摘　　要：采用水浴回流法提取灵芝孢子粉粗多糖,再依次使用DEAE Sepharose Fast Flow离子交换柱和Sepharose CL-6B凝胶色谱柱分离纯化得到2种多糖组分GLP1a和GLP1b,HPSEC法测得2种组分均呈单一峰,重均分子量分别为1.12×106和1.21×105。HPLC、红外光谱和核磁共振分析的结果表明,GLP1a和GLP1b主要由葡萄糖组成,主链都是以β-(1,6)糖苷键连接,GLP1b在部分1→6连接的葡萄糖的3位和4位有分支。MTT实验表明,2个多糖级分均具有一定的免疫活性,而且都存在明显的量效关系,GLP1b的免疫活性明显高于GLP1a。The crude polysaccharides was obtained from the hot-water extract of the spores of Ganoderma lucidum,and two main fractions GLP1 a and GLP1 b were isolated by DEAE Sepharose Fast Flow chromatography and Sepharose CL-6 B gel chromatography. The HPSEC analysis indicated that the two fractions were all homogeneous polysaccharides and their velative molecular mass weight were respectively 1.12 ×10^6 and 1.21 ×10^5. The analysis of composition,infrared spectrum and NMR spectroscopy indicated that GLP1 a and GLP1 b all had a backbone of β-D-（1,6）-glucan and GLP1 b were partly substituted at C-3 and C-4 in the main chain. Preliminary tests in vitro showed that the two polysaccharide fractions all has stimulating effects on murine splenocyte proliferation and the effect of GLP1 b was better than GLP1 a. Moreover,their activities were all in a dose-dependent manner. GLP1 a.

关 键 词：灵芝孢子粉多糖 分离纯化 结构表征 免疫活性 

分 类 号：Q539[生物学—生物化学]