大鼠热应激对细胞免疫学的影响  

The effection of heat stress on cell immunology in rats

作　　者：周文芸[1] 吕芷娴 曾黎峰[1] 何丹[1] 胡国柱[1] ZHOU Wenyun;LV Zhixian;ZENG Lifeng;HE Dan;HU Guozhu(Institution of Clinical Medicine,People′s Hospital of Jiangxi Provine,Nanchang,Jiangxi 330006,Chin)

机构地区：[1]江西省人民医院临床医学研究所,南昌330006

出　　处：《重庆医学》2018年第17期2270-2274,共5页Chongqing medicine

摘　　要：目的探讨热应激对大鼠脾脏细胞Toll样受体4(TLR4)及外周血T调节(Treg)细胞表达的影响。方法将72只SD大鼠分为20℃常温组和37℃高温湿热组,每组分非刺激、细菌脂多糖(LPS)刺激、刀豆素-A(Con-A)刺激亚组,每个亚组设1、12、48、168h观察点;流式细胞术测定TLR4+细胞及Treg细胞。结果37℃高温湿热组脾脏TLR4+细胞比率在各亚组的1~168h均低于20℃常温组(P<0.05)。37℃高温湿热组各亚组1~48h的外周血CD4+CD25+Treg较20℃常温组显著下降(P<0.05),只有LPS刺激的168h稍有升高,37℃高温湿热组中非刺激及Con-A刺激亚组CD4+CD25+Foxp3+Treg在12h时间点较20℃常温组显著升高,而在168h时间点各亚组显著降低(P<0.05)。37℃高温湿热组中各亚组1h和168h的外周血CD8+CD25+Treg较20℃常温组显著升高;而CD8+CD25+Foxp3+Treg各亚组在1h和48h较20℃常温组显著降低,在非刺激和LPS刺激亚组的12h和168h较20℃常温组显著升高(P<0.05)。结论高温湿热可破坏大鼠固有免疫并改变适应性免疫的功能状态。Objective To investigate the effect of heat stress on the expression of Toll-like receptor 4 （TLR4） and peripheral blood T regulatory cells （Treg） in splenic cells of rats. Methods Senventy-two SD rats were divided into 20 ℃ control group and 37 ℃ group.Each group was divided into non stimulation,bacterial lipopolysaccharide （LPS） stimulation and concanavin A （Con-A） stimulation subgroup.Each subgroup had 1,12,48 h and 168 h observation points,and flow cytometry was used to determine the level of TLR4 and Treg. Results The TLR4 ＋ immunocompetent cells in the spleen was decreased from 1 h to 168 h in every subgroup of the 37 ℃ group compared to the 20 ℃ control group （ P 〈0.05）.The level of CD4 ＋CD25 ＋ Treg in the peripheral blood in rats from 1 to 48 h was significantly decreased （ P 〈0.05） and slightly increased at 168 h in LPS stimulation subgroup in the 37 ℃ group compared to the 20 ℃ control group.The level of CD4 ＋CD25 ＋Foxp3 ＋ Treg in the peripheral blood in rats at 12 h were significantly increased in non-stimulation and concanavalin A （Con-A） stimulation subgroups in the 37 ℃group,and were significantly decreased at 168 h in every subgroup of the 37 ℃ group compared with 20 ℃ control group （ P 〈0.05）.The level of CD8 ＋CD25 ＋Treg in the peripheral blood in rats was significantly increased at 1 h and 168 h in the 37 ℃ hot and humid group in the every subgroup when compared with the 20 ℃ control group （ P 〈0.05）.The level of CD8 ＋CD25 ＋Foxp3 ＋ Treg in the peripheral blood in rats was significantly decreased at 1 h and 48 h in the every subgroup,and was significantly increased at 12 h and 168 h in the non-stimulation and LPS stimulation subgroups in the 37 ℃ group compared to the 20 ℃ control group （ P 〈0.05）. Conclusion High temperature and damp heat can destroy the innate immunity and alter the functional state of adaptive immunity in rats.

关 键 词：SD大鼠 脾脏 免疫细胞 TLR4 TREG 热应激 LPS Con-A 

分 类 号：R363[医药卫生—病理学]