具有抗菌活性的水杉内生真菌的分离及筛选  

Isolation and Screening of Endophytic Fungi with Antimicrobial Activity in Metasequoia

作　　者：张垚 白金峰 徐凯莉 刘宁[1] ZHANG Yaol;BAI Jin-fengl;XU Kai-li;LIU Ning(College of Life Sciences,Hebei Agriculture University,Baoding 071001,Hebei,China;College of Chemistry and Pharmaceutical Engineering,Hebei University of Science ＆ Technology,Shijiazhuang 050018,China)

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北科技大学化学与制药工程学院,石家庄050018

出　　处：《湖北农业科学》2018年第11期36-38,41,共4页Hubei Agricultural Sciences

基　　金：河北农业大学教改项目(2015JM04);河北省教育厅课题"生物技术"品牌特色专业建设研究(2012GJJG054);教育部;河北省教育厅"生物技术"专业综合改革试点项目(201202)

摘　　要：从水杉(Metasequoia glyptostroboides Hu et Cheng)树皮、叶片中分离筛选得到了17株形态各不相同的内生真菌,采用对峙培养法筛选对植物病原真菌,层出镰孢菌(Fusarium proliferatum)﹑禾谷镰刀菌(Fusarium graminearum)﹑玉米大斑病菌(Setosphaeria turcica)﹑玉米小斑病菌(Helminthosporium maydis)具有拮抗作用的菌株,筛选得到了1株对以上4种病原菌具有广谱抗菌活性的菌株,命名为SS-17。通过对SS-17菌株的形态特征观察及ITS分子鉴定,初步鉴定为黑附球菌(Epicoccum nigrum)。In this research,17 strains of the endophytic fungi were separated from the leaves and bark of Metasequoia glyptostroboides with different colony morphology,and the strains with antagonistic action were screened by dual culture method against plant pathogenic fungi including Fusarium proliferatum,F. graminearum,Setosphaeria turcica,Helminthosporium maydis. 1 strain was obtaind with antagonism to these strains above,named SS-17. With the morphology and ITS molecular identification of SS-17 strain,it was identified as Epicoccum nigrum.

关 键 词：水杉(Metasequoia glyptostroboides HU et Cheng) 内生真菌 筛选 抑菌活性 黑附球菌(Epicoccum nigrum) 

分 类 号：Q939.92[生物学—微生物学]