球孢白僵菌菌丝体成分及抗氧化活性研究  

Component Analysis and Antioxidant Activity of Beauveria bassiana Mycelia

作　　者：邵颖[1,2] 任格 孙颖 陈安徽[1,2] SHAO Ying 1,2 , REN Ge 1, SUN Ying 1 ,et al(1.College of Food （Biological） Engineering, Xuzhou University of Technology, Xuzhou,Jiangsu 221111;2. Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou,Jiangsu 221111)

机构地区：[1]徐州工程学院食品(生物)工程学院,江苏徐州221111 [2]江苏省食品资源开发与质量安全重点建设实验室徐州工程学院,江苏徐州221111

出　　处：《安徽农业科学》2018年第18期160-162,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省大学生创新创业计划项目(201611998033Y);江苏省高校自然科学研究重大项目(15KJA180008);徐州工程学院大学生创新创业基金项目(201610)

摘　　要：[目的]研究球孢白僵菌菌丝体的化学成分及抗氧化活性。[方法]利用直接灰分法、苯酚-硫酸法、3-5二硝基水杨酸法、索氏抽提法、酸解法对球孢白僵菌菌丝体的灰分、总糖、还原糖、粗脂肪及氨基酸进行分析。采用水提醇沉法提取了球孢白僵菌菌丝体中的粗多糖,通过构建铁离子螯合能力、DPPH自由基清除活性、还原力3个抗氧化模型,研究菌丝体粗多糖的抗氧化活性。[结果]球孢白僵菌菌丝体样品的灰分、总糖、还原糖、粗脂肪含量分别为6.61%、2.44%、0.90%、5.23%,菌丝体中含有7种必需氨基酸,占所测定的18种氨基酸总量的39.94%,必需氨基酸与非必需氨基酸的比值为66.50%。同时,菌丝体粗多糖显示了一定的抗氧化活性。[结论]球孢白僵菌菌丝体中营养成分较全面,并具有一定的抗氧化活性。[Objective] The research aimed to study the component analysis and antioxidant activity of Beauveria bassiana mycelia.[Method]The contents of ash,total sugar,reducing sugar,fat and amino acids of Beauveria bassiana mycelia were analyzed by direct ash method, phenol-sulfuric acid method, 3-5 dinitrosaliylic acid method, Soxhlet extraction method and acidolysis method.Polysaccharides from Beauveria bassiana mycelia were extracted by water extraction and alcohol precipitation.Three antioxidant models established by chelating iron ions, scavenging DPPH radicals and reducing power were to study antioxidant activity of polysaccharides from mycelium. [Result]The contents of ash,total sugar,reducing sugar,fat in Beauveria bassiana mycelia were 6.61%, 2.44%, 0.90%, 5.23%. The seven essential amino acids account for 39.94% of the total amount of eighteen amino acids and the ratio of essential amino acids to the non-essential amino acid was 66.50%. At the same time,polysaccharides of the mycelia had certain effect on the three antioxidant models. [Conclusion]The nutritional value of Beauveria bassiana mycelia was more comprehensive and Beauveria bassiana mycelia had certain antioxidant activity.

关 键 词：球孢白僵菌 菌丝体 成分 抗氧化活性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]