生长分化因子15基因的克隆及重组原核表达载体的构建  

Cloning of Human Growth Differentiation Factor 15 Gene and Construction of Recombinant Prokaryotic Expression Vector

作　　者：董艳敏 刘满宇 张文慧[1,2] 张林波[1,2] Dong Yanmin(College of Life Science,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118 [2]生物反应器与药物开发教育部工程研究中心,吉林长春130118

出　　处：《安徽农学通报》2018年第11期13-15,共3页Anhui Agricultural Science Bulletin

基　　金：吉林省教育厅"十三五"科学技术项(2016-177)

摘　　要：为克隆人生长分化因子15基因,构建h GDF15基因的重组原核表达载体,为研究该基因的功能奠定实验基础,利用PCR技术克隆其基因序列,将其分别连接至p ET28a和p Cold I载体,构建重组p ET28a-h GDF15和p Cold I-h GDF15原核表达载体,并进行质粒PCR和双酶切验证以及测序验证。结果表明:重组载体构建成功,为进一步研究h GDF15蛋白的作用机制奠定基础。For gene clone growth differentiation factor 15,constructing h GDF 15 gene recombinant prokaryotic expression vector,which laid the foundation for the study of the function of this gene,cloning thegene sequences using the PCR technology. It＇s connection to p ET28 a and p Cold I carrier respectively,construct recombinant p ET28 a h GDF15 and p Cold I h GDF15 prokaryotic expression vector,and verified plas-mid PCR and double enzyme digestion and sequencing.The results show that the method of recombinant carrier is successful,which lays a foundation for further research on the mechanism of h GDF15 protein.

关 键 词：人生长分化因子15 原核载体 

分 类 号：Q78[生物学—分子生物学]