含caspase募集区的凋亡抑制蛋白ARC在卵巢癌细胞中的表达及其对内源性凋亡的调节  

The apoptosis repressor with caspase recruitment domain( ARC) impedes intrinsic apoptotic pathway in ovarian cancer cells

作　　者：朱静[1,2] 孙红[1] 朱杰[1,2] 郑亚[1,2] Zhu Jing;Sun Hong;Zhu Jie(Shanghai Obstetrics and Gynecology Hospital,Fudan University,Shanghai 200090;Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases,Fudan University,Shanghai 200011)

机构地区：[1]复旦大学附属妇产科医院,上海200090 [2]复旦大学上海市女性生殖内分泌相关疾病重点实验室,上海200011

出　　处：《现代妇产科进展》2018年第6期414-417,共4页Progress in Obstetrics and Gynecology

基　　金：上海市科研计划项目资助(No:15ZR1404800)

摘　　要：目的:探讨含casepase募集区的凋亡抑制蛋白ARC在卵巢癌细胞株中的表达,以及其对卵巢癌细胞凋亡的影响及内源性凋亡蛋白的调节。方法:脂质体转染干扰卵巢癌细胞A2780和SKOV3细胞内ARC表达。Western blot和RT-PCR法检测细胞内ARC及其编码基因NOL3,流式细胞仪检测细胞凋亡比例,观察细胞内Bax和Bcl-xl表达。结果:卵巢癌细胞株中ARC均为高表达或较高表达,A2780和SKOV3细胞内ARC表达减少使细胞的凋亡比例增加,导致Bax表达增加,Bcl-xl表达减少。结论:含caspase募集区的凋亡抑制蛋白ARC可通过内源性凋亡途径抑制卵巢癌细胞凋亡。Objective： To investigate the expression of apoptosis repressor with caspase recruitment domain（ ARC） and the role of ARC played in intrinsic apoptotic pathway in ovarian cancer cells. Methods： A2780 and SKOV3 cells were transfected with an ARC-encoding gene NOL3 targeting siRNA plasmid（ p Gp U6-NOL3）. An empty vector（ p Gp U6-NC） were used as an experimental control. Western blot and RT-PCR was applied to examine the expression of ARC and Bax,Bcl-xl.Apoptotic cells were detected by flow cytometry.Results： High positive expression of ARC was detected in ovarian cancer cell lines. Down-regulation of ARC by using plasmid p Gp U6-NOL3 was achieved in A2780 and SKOV3 cells. The apoptotic cells ratio and Bax protein expression enhanced and Bcl-xl protein expression depressed following down-regulation of ARC. Conclusion： ARC shows high expression and plays a part in manipulating mitochondria apoptosis in ovarian cancer cells.

关 键 词：ARC 卵巢癌 凋亡 Bax BCL-XL 

分 类 号：R737.33[医药卫生—肿瘤]