白藜芦醇调节SphK1/AP-1信号通路抑制高糖诱导的肾小球系膜细胞增殖  

Effects of resveratrol on cell proliferation in glomerular mesangial cells cultured under high glucose by inhibiting SphK1/AP-1 signal pathway

作　　者：邓艳辉 公文艳 李强 伍飞臻 段萍萍 黄河清[2] DENG Yan-hui;GONG Wen-yan;LI Qiang;WU Fei-zhen;DUAN Ping-ping;HUANG He-qing(Dept of Pharmacy,the Third Affiliated Hospital of Southern Medical Universit;Lab of Pharmacology and Toxicology,School of Pharmaceutical Sciences,Sun Yat-Sen Universit)

机构地区：[1]南方医科大学第三附属医院药学部,广东广州510630 [2]中山大学药学院药理毒理实验室,广东广州510006

出　　处：《中国药理学通报》2018年第7期959-963,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81603355);广东省中医药管理局项目(No 20161154)

摘　　要：目的观察白藜芦醇干预对高糖培养的肾小球系膜细胞增殖成分纤维连接蛋白(FN)、TGF-β1表达的影响,并进一步探讨其对SphK1/AP-1信号通路的调节作用。方法采用高糖诱导的大鼠肾小球系膜细胞株,MTT测定白藜芦醇对肾小球系膜细胞的生存活力及增殖的影响;Western blot测定FN、TGF-β1、SphK1蛋白的表达;EMSA测定AP-1的活性。结果白藜芦醇可呈浓度依赖性地抑制高糖诱导的肾小球系膜细胞增殖,降低肾小球系膜细胞中SphK1表达,抑制AP-1活性,从而抑制FN、TGF-β1表达。结论白藜芦醇抑制高糖诱导的肾小球系膜细胞增殖,该作用可能与其抑制SphK1/AP-1信号通路密切相关。Aim To observe the expression of FN and TGF-β1 in the glomerular mesangial cells induced by high-glucose after the intervention of resveratrol,and further discuss its influence on SphK1/AP-1 signaling pathway.Methods The rat glomerular mesangial cells induced by high glucose were used to observe the effects of resveratrol on cell proliferation after intervention.The survival vitality and proliferation of glomerular mesangial cells were determined by MTT,and then FN,TGF-β1 and SphK1 protein expression were determined by Western blot.Also,AP-1 activity was determined by EMSA assay.Results Resveratrol could obviously inhibit the proliferation of high glucose-induced glomerular mesangial cells,lower SphK1 expression,inhibit AP-1 activity and thus inhibit the expression of FN,TGF-β1.Conclusions Resveratrol inhibits the proliferation of high glucose-induced glomerular mesangial cells,which may be closely related to the inhibition of SphK1/AP-1 signaling pathway.

关 键 词：白藜芦醇 糖尿病肾病 细胞增殖 SphK1/AP-1信号通路 纤维连接蛋白 转化生长因子Β1 

分 类 号：R-332[医药卫生] R284.1