草氨酸钠抑制乏氧鼻咽癌细胞CNE2血管紧张素Ⅱ生成提高放射敏感性  

Role of sodium oxalate in radiosensitivity of hypoxic NPC CNE2 cells

作　　者：程坦[1] 郑杰灵 刘颖[1] 谢国柱[1] 袁亚维[1] CHENG Tan, ZHENG Jieling, LIU Ying , XIE Guozhu, YU-AN Yawei.(Department of Radiation Oncology , Nanfang Hospital, Southern Medical University, Guangzhou 510515, Chin)

机构地区：[1]南方医科大学南方医院放疗科,广州510515

出　　处：《实用医学杂志》2018年第12期1934-1937,共4页The Journal of Practical Medicine

基　　金：2016年国家自然科学基金(编号:81672633);2017年广东省科技计划项目(编号:2017A020215171)

摘　　要：目的探讨草氨酸钠对鼻咽癌细胞放射敏感性的影响。方法利用乳酸测定试剂盒和血管紧张素ⅡELISA试剂盒检测草氨酸钠对鼻咽癌细胞株CNE2乳酸和血管紧张素Ⅱ生成的影响;应用MTT细胞增殖实验检测草氨酸钠对乏氧CNE2细胞增殖能力的影响;利用克隆形成实验检测草氨酸钠对乏氧CNE2鼻咽癌细胞放射敏感性的影响。结果草氨酸钠处理后CNE2细胞的乳酸以及血管紧张素Ⅱ生成减少,草氨酸钠有抑制乏氧CNE2细胞增殖的作用。草氨酸钠处理的乏氧CNE2细胞对射线的敏感度明显增加。结论草氨酸钠对乏氧CNE2鼻咽癌细胞有增殖抑制作用并显著提高了其放射敏感性,其机制可能与其抑制了CNE2细胞血管紧张素Ⅱ的生成有关。Objective To explore the influence of sodium oxamate on the radiaosensitivity of hypoxicnasopharyngeal carcinoma CNE2 cells. Methods The production of lactate and angiotensin Ⅱ in CNE2 with orwithout sodium oxamate treatment was detected by lactate assay kit and angiotensin Ⅱ ELISA kit. Cell growth ofhypoxic CNE2 with or without sodium oxamate treatment was measured in vitro by MTT method. Radiosensitivity ofhypoxic CNE2 with or without sodium oxamate treatment was tested by clone formation assay. Results Lactate andangiotensin Ⅱ production of CNE2 cells treated with sodium oxalate was inhibited. Sodium oxalate inhibited theproliferation of hypoxic CNE2 cells. The radiosensitivity of hypoxic CNE2 cells treated with sodium oxalate waslargely improved. Conclusions Sodium oxalate inhibits the proliferation of CNE2 cells in hypoxic condition andsignificantly enhances the radiosensitivity of hypoxic CNE2 cells. The mechanism may be involved in its inhibitingof angiotensin Ⅱ production.

关 键 词：鼻咽癌 乏氧 草氨酸钠 放射敏感性 

分 类 号：R739.63[医药卫生—肿瘤]