急性髓系白血病患者miR-372的表达水平及其临床意义  

Expression and mechanism of plasma miR-372 in acute myeloid leukemia patients

作　　者：赵强强[1] 冯建明[1] 李文倩[1] 沈括[1] 陈绍斌[1] 解友邦[1] 赵长明 侯艳 ZHAO Qiangqiang, FENG Jianming, LI Wenqian, SHEN Kuo, CHEN Shaobin, XIE Youbang, ZHAO Changming, HOU Yan(Department of Hematology, Qinghai People＇s Hospital, Xining 810007, Chin)

机构地区：[1]青海省人民医院血液科,西宁810007 [2]青海大学,西宁810016

出　　处：《实用医学杂志》2018年第12期2030-2034,共5页The Journal of Practical Medicine

基　　金：2014年度青海省人才"小高地"项目(编号:青人才字[2014]12号);2011年度国家临床重点专科建设项目(编号:卫办医政函[2011]873号)

摘　　要：目的探讨miR-372在急性髓系白血病(AML)患者的血浆中的表达情况及其参与AML发病的可能作用机制。方法实时定量PCR检测血浆中miR-372的水平。生物信息学软件预测miR-372可能的靶基因并通过双荧光素酶报告基因验证。在HL-60细胞中,敲低miR-372,通过划痕实验和克隆形成实验检测其对细胞迁移和克隆形成的影响。结果 AML患者血浆中miR-372的水平显著上调。受试者工作特征(ROC)曲线分析表明,miR-372可以区分AML患者以及健康对照组。双荧光素酶报告基因结果表明,miR-372可以抑制PTEN-3′UTR的活力。在HL-60细胞中敲低miR-372可以显著降低HL-60的细胞迁移率和克隆形成能力。结论在急性髓系白血病患者血浆中,miR-372水平显著升高。通过靶向抑癌基因PTEN,miR-372可能成为一个潜在的筛查和诊断急性髓系白血病的非侵入性生物学标志物。Objective To investigate the expression of miR-372 in the plasma of patients with acute my-eloid leukemia（AML）and the possible mechanism to participate in the development of AML. Methods Real-time quantitative PCR was used to detect the level of miR-372 in plasma. Bioinformatics software predicted the pos-sible target genes of miR-372 and dual luciferase reporter assay was performed to validate the prediction. In HL-60 cells,miR-372 was knocked down,and the effects on cell migration and cloning were detected by scratch test andclone formation. Results The level of miR-372 was significantly up-regulated in the plasma of AML patients. ROCanalysis showed that miR-372 could distinguish between AML patients and healthy controls. Dual luciferase report-er assay showed that miR-372 could inhibit the activity of PTEN-3′UTR. Inhibition of miR-372 in HL-60 cells cansignificantly reduce the cell migration rate and clone formation ability. Conclusion In summary,for the firsttime,we showed novel data that the level of miR-372 was increased in the plasma of AML patients. By targetingthe tumor suppressor gene PTEN,miR-372 may become a potential noninvasive biomarker for the screening and di-agnosis of AML.

关 键 词：miR-372 血浆 急性髓系白血病 PTEN 

分 类 号：R733.71[医药卫生—肿瘤]