白细胞介素22诱导胃癌细胞AGS分泌VEGF-A的机制研究  

The mechanism of interleukin-22 induced the secretion of vascular endothelial growth factor A in gastric cancer cell line AGS

作　　者：罗秀英 钟巧玲[1] LUO Xiuying;ZHONG Qiaoling(Department of Clinical Laboratory,the Second People′s Hospital of Banan District,Chongqing 400054,Chin)

机构地区：[1]重庆市巴南区第二人民医院检验科,重庆400054

出　　处：《国际检验医学杂志》2018年第13期1547-1549,1552,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨白细胞介素22(IL-22)诱导胃癌细胞AGS分泌血管内皮生长因子A(VEGF-A)的分子机制。方法 IL-22刺激体外培养的胃癌细胞系AGS细胞,或预先加入不同的信号通路抑制剂作用1h后再利用IL-22进行刺激,酶联免疫吸附试验检测细胞培养上清液中VEGF-A的水平。结果 AGS细胞经IL-22刺激后,VEGF-A的分泌显著增加,差异有统计学意义(P<0.05),且具有剂量和时间依赖性;加入信号传导转录激活因子3(STAT3)抑制剂预孵育后,IL-22诱导AGS细胞分泌的VEGF-A水平显著下降,差异有统计学意义(P<0.05)。但核因子-κB(NF-κB)、c-Jun氨基末端激酶(JNK)、丝裂原活化细胞外信号调节激酶1/2(MEK1/2)和p38丝裂原激活蛋白激酶(p38/MAPK)抑制剂预孵育的VEGF-A水平比较差异无统计学意义(P>0.05)。结论 IL-22可能经STAT3信号通路诱导AGS细胞分泌VEGF-A,从而有助于胃癌的进展。Objective To investigate the mechanism of interleukin-22（IL-22）induced the secretion of vascular endothelial growth factor A（VEGF-A）in gastric cancer cell line AGS.Methods Gastric cancer cell line AGS were cultured in vitro,and recombination cytokine IL-22 were added,or signal pathway inhibitor were pre-incubated with AGS for 1 hour and then IL-22 were added,the level of VEGF-A were detected by enzymelinked immunosorbent assay.Results Compared with the unstimulated group,the secretion of VEGF-A in IL-22-stimulated group was significantly increased,the difference was statistically significant（P〈0.05）,which was in a dose and time dependent manner.In addition,IL-22-stimulated the secretion of VEGF-A by AGS was significantly decreased while pre-incubated by the signal transducers and activators of transcription 3（STAT3）inhibitor,the difference was statistically significant（P〈0.05）,but such effect was not observed while AGS were pre-incubated with the nuclear factor kappa B inhibitor,c-Jun N-terminal kinase inhibitor,mitogen-activated protein kinase kinase 1/2 inhibitor and p38 mitogen-activated protein kinase inhibitor,there was no statistical significance（P〉0.05）.Conclusion IL-22 could induce the secretion of VEGF-A in gastric cancer cell line AGS via STAT3 signal pathway,which may contribute to tumor progression.

关 键 词：白细胞介素22 血管内皮生长因子A 信号传导转录激活因子3 

分 类 号：R735.2[医药卫生—肿瘤]