甘蓝型油菜组培苗生根培养体系的优化  

Optimization of rooting medium for in vitro transgenic shoots in Brassica napus

作　　者：郝梦宇 丁炳莉 李超 付丽[1] 胡琼[1] 成洪涛 HAO Meng-yu;DING Bing-li;LI Chao;FU Li;HU Qiong;CHENG Hong-tao(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences/National Center of Oil Crops Improvement,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,Chin)

机构地区：[1]中国农业科学院油料作物研究所/国家油料作物改良中心/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2018年第3期352-358,共7页Chinese Journal of Oil Crop Sciences

基　　金：中国农业科学院科技创新工程项目(Group No.118);国家科技支撑计划(2014BAD1807);国家农业现代产业技术体系建设专项(CARS-13);湖北省科技创新工程(201620000001048)

摘　　要：为解决油菜组织培养中组培苗生根细短、移栽不易成活的问题,以甘蓝型半冬性油菜转化p CAMBIA1301空载体的再生植株为材料,以生根时间、根长、根重、苗高、苗重、总重、生根数、生根率等指标,评价以MS和B5为基础培养基,分别使用琼脂糖、琼脂粉及植物凝胶为凝固剂时再生苗的生根效率,得到了两种基础培养基的最适凝固剂组合;在以上两种组合的培养基中分别加入不同浓度蔗糖,比较生根情况和成本,认为含20g/L蔗糖的B5琼脂粉培养基为最优配方。利用优化后的生根培养基配方,可以极大地促进油菜组培苗的生根长度和数目,提高后期移栽成活率。In rapeseed tissue culture,tenuous and short roots lead to low survival rate of transplanted. To modify the rooting system and to ensure related study,shoots regenerated from a semi-winter oilseed rape cultivar transformed with empty p CAMBIA1301 vector were used. Rooting time,root length,shoot height,shoot weight and plantlet biomass were investigated to assess rooting efficiency of basal medium MS and B5 combined with agar,agarose and phytagel as gelling agent. Results showed that,considering the cost,B5 agar medium containing 20 g/L sucrose were the optimal formula. The optimized rooting medium formula could increase root length and number of in vitro plantlets,and thus enhance the survival rate after transplanting.

关 键 词：甘蓝型油菜 组织培养 生根培养 凝固剂 蔗糖 

分 类 号：S565.4[农业科学—作物学]