淫羊藿化学成分的分离与鉴定  

Isolation and Identification of Chemical Constituents of Herba Epimedii

作　　者：黄楚燕 梁宏宇[2] Huang Chuyan;Liang Hongyu(Department of Pharmacy,The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Second Department of Cardiovascular Diseases,Guangdong Hospital of Traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学第一附属医院药学部门诊中药房,广州510405 [2]广东省第二中医院心脑血管二区,广州510405

出　　处：《世界中医药》2018年第5期1262-1265,共4页World Chinese Medicine

基　　金：广东省中医药局科研项目(2010445)

摘　　要：目的:探讨淫羊藿叶的化学组成成分。方法:使用萃取、正相硅胶、溶剂法提取法、制备高效液相色谱分析法、Sephadex LH-20、反相ODS等多种分离纯化方法,来对淫羊藿叶化学成分进行分离研究,综合应用磁共振成像、质谱等方法鉴定各单体化合物结构。结果:从淫羊藿干燥叶的95%乙醇提取物(V/V)中共分离鉴定7个化合物,分别为4-羟基肉桂酸甲酯;4-羟基-3-异戊二烯基-苯甲酸;3,5,7,4'-四甲氧基黄酮;2,3,4,6,7-五甲氧基-菲;5,7,4'-三甲氧基-4-苯基香豆素;4-羟基-(2E)-2-壬烯酸;4,5,-二羟基-2,3,6-三甲氧基-9,10-二氢菲。结论:化合物1-7均为首次从淫羊藿属分离得到化合物。Objective：To study chemical composition of Epimedium leaves. Methods：The extraction method, silica gel, extrac-tion solvent, preparation of high performance liquid chromatography, Sephadex LH-20, ODS and other purification methods were used to study the separation of chemical constituents of the leaves of epimedium. NMR and mass spectrometry were comprehensive-ly applied to identify-all the compounds. Results ：7 compounds were isolated and identified from the 95 % ethanol extract of Epime-dium dry leaves （ V/V）, including （ 1 ） 4-hydroxy cinnamic acid methyl ester, （2） 4-hydroxy-3-polyisoprene-henzoic acid, （ 3 ） 3,5,7,4＇ tetramethoxflavone, （ 4 ） 2,3,4,6,7-five methoxy-Philippines, （ 5 ） 5,7,4 ＇-trimethoxy-4-phenylcoumarin, （ 6 ） 4 - （ 2 E ）- 2-hydroxy-nonenoic acid, （7） 4,5, dihydroxyl-2,3,6-trimethoxy-9,10-dihydrophenanthrene. Conclusion ： Compound 1-7 is firstly isolated from Epimedium genus compounds.

关 键 词：化学成分 淫羊藿 结构 鉴定 

分 类 号：R735.704[医药卫生—肿瘤]